用于活细胞中 ClO 和 NH 区分检测的双分析物响应荧光探针。
A dual-analytes responsive fluorescent probe for discriminative detection of ClO and NH in living cells.
机构信息
School of Food and Drug, Luoyang Normal University, Luoyang 471934, PR China; Institute of Veterinary Drug, Jiangxi Agricultural University, Nanchang 330045, PR China.
School of Food and Drug, Luoyang Normal University, Luoyang 471934, PR China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Feb 5;246:118953. doi: 10.1016/j.saa.2020.118953. Epub 2020 Sep 17.
Hydrazine (NH) and ClO are very harmful for public health, hence it is important and necessary to monitor them in living cells. Herein, we rationally designed and synthesized a dual-analytes responsive fluorescent sensor PTMQ for distinguishing detection of NH and ClO. PTMQ underwent NH-induced double bond cleavage, affording colorimetric and green fluorescence enhancement with good selectivity and a low detection limit (89nM). On the other hand, PTMQ underwent ClO-induced sulfur oxidation and displayed red fluorescence lighting-up response towards ClO with good selectivity, rapid response (<0.2min) and a low detection limit (58nM). Moreover, PTMQ was successfully employed for in-situ imaging of NH and ClO in living cells.
肼（NH）和 ClO 对公众健康非常有害，因此监测它们在活细胞中的含量非常重要和必要。在此，我们合理设计并合成了一种双分析物响应荧光传感器 PTMQ，用于区分检测 NH 和 ClO。PTMQ 经历了 NH 诱导的双键断裂，提供了比色和绿色荧光增强，具有良好的选择性和低检测限（89nM）。另一方面，PTMQ 经历了 ClO 诱导的硫氧化，并且对 ClO 显示出红色荧光点亮响应，具有良好的选择性、快速响应（<0.2min）和低检测限（58nM）。此外，PTMQ 成功地用于活细胞中 NH 和 ClO 的原位成像。
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