Department of Animal Biotechnology and Genetics, UTP University of Science and Technology, Mazowiecka 28 St, 85-084 Bydgoszcz, Poland.
Department of Meat and Fat Technology, Prof. Wacław Dąbrowski Institute of Agricultural and Food Biotechnology, Rakowiecka 36 St., 02-532 Warsaw, Poland.
Meat Sci. 2021 Feb;172:108314. doi: 10.1016/j.meatsci.2020.108314. Epub 2020 Sep 18.
This study aimed to genotype the polymorphism (c.654G > A) in the exon 3 of the insulin-like growth factor 1 receptor gene (IGF1R) and to analyze its association with growth, body size, slaughter and meat quality traits in Colored Polish Merino sheep. In total, 67 traits were analyzed. The IGF1R polymorphism was genotyped using the polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) method. The MIXED procedure of the SAS software was used to assess the genotypic effects of the polymorphism (c.654G > A) on production traits of interest. The IGF1R c.654G > A genotypes were found to have a significant effect on the average daily gain between the 56th and 78th day of life, cold carcass, leg part, leg cut, fore shank, and kidney weights, as well as eye of loin depth, intramuscular fat content, and water-holding capacity of meat. The results suggest that the studied polymorphism may provide useful information for marker-assisted selection for increased meat performance in Colored Polish Merino sheep.
本研究旨在对波兰花毛羊胰岛素样生长因子 1 受体基因（IGF1R）外显子 3 中的多态性（c.654G>A）进行基因分型，并分析其与生长、体型、屠宰和肉质性状的关系。共分析了 67 个性状。采用聚合酶链反应单链构象多态性（PCR-SSCP）方法对 IGF1R 多态性进行基因分型。使用 SAS 软件的 MIXED 过程评估多态性（c.654G>A）对感兴趣的生产性状的基因型效应。结果表明，IGF1R c.654G>A 基因型对 56 至 78 日龄的平均日增重、冷胴体、腿部、腿部切块、前腿和肾脏重量以及腰眼深度、肌内脂肪含量和肉的持水力有显著影响。研究结果表明，该研究的多态性可能为波兰花毛羊增加肉质性能的标记辅助选择提供有用信息。
