Barker D G, Johnston L H
Eur J Biochem. 1983 Aug 1;134(2):315-9. doi: 10.1111/j.1432-1033.1983.tb07568.x.
The Saccharomyces cerevisiae cdc9 gene has been cloned in the vector YRp12 by complementation of the temperature-sensitive lesion in vivo. The gene is contained within a 3300-base-pair fragment of DNA, which maps to the chromosomal locus of cdc9 and which is able to complement a DNA-ligase-deficient mutant of the fission yeast Schizosaccharomyces pombe.
通过在体内对温度敏感损伤进行互补,酿酒酵母的cdc9基因已被克隆到载体YRp12中。该基因包含在一段3300个碱基对的DNA片段内,该片段定位于cdc9的染色体位点,并且能够互补粟酒裂殖酵母的DNA连接酶缺陷型突变体。