Estupiñan Oscar R, Garcia-Manrique Pablo, Blanco-Lopez Maria Del Carmen, Matos Maria, Gutiérrez Gemma
Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), Hospital Universitario Central de Asturias, 33006 Oviedo, Spain.
CIBER en Oncología (CIBERONC), 28029 Madrid, Spain.
Foods. 2020 Sep 26;9(10):1367. doi: 10.3390/foods9101367.
Vesicular nanocarriers have an important role in drug delivery and dietary supplements. Size control and optimization of encapsulation efficiency (EE) should be optimized for those applications. In this work, we report on the identification of the crucial step (injection, evaporation, or sonication) innanovesicles (transfersomes and niosomes) preparation by theethanol injection method (EI). The identification of each production step on the final vesicle size was analyzed in order to optimize further scale-up process. Results indicated that the final size of transfersomeswas clearly influenced by the sonication step while the final size of niosomes was mainly governed by the injection step. Measurements of final surface tension of the different vesicular systems prepared indicate a linear positive tendency with the vesicle size formed. This relation could help to better understand the process and design a vesicular size prediction model for EI. Vitamin D3 (VitD3) was encapsulated in the systems formulated with encapsulation efficiencies larger than 90%. Interaction between the encapsulated compound and the membrane layer components is crucial for vesicle stability. This work has an impact on the scaling-up production of vesicles for further food science applications.
囊泡纳米载体在药物递送和膳食补充剂中具有重要作用。对于这些应用，应优化尺寸控制和包封效率（EE）。在这项工作中，我们报告了通过乙醇注入法（EI）制备纳米囊泡（传递体和非离子型表面活性剂囊泡）时关键步骤（注射、蒸发或超声处理）的识别。分析了每个生产步骤对最终囊泡尺寸的影响，以便进一步优化放大过程。结果表明，传递体的最终尺寸明显受超声处理步骤的影响，而非离子型表面活性剂囊泡的最终尺寸主要由注射步骤决定。对所制备的不同囊泡系统的最终表面张力测量表明，其与所形成的囊泡尺寸呈线性正相关趋势。这种关系有助于更好地理解该过程，并为EI设计一个囊泡尺寸预测模型。维生素D3（VitD3）被包封在包封效率大于90%的系统中。包封化合物与膜层成分之间的相互作用对囊泡稳定性至关重要。这项工作对进一步扩大用于食品科学应用的囊泡生产规模具有重要意义。
