Faculty of Pharmaceutical Sciences, Government College University Faisalabad, Faisalabad 38000, Pakistan.
Institute of Industrial Biotechnology, Government College University Lahore, Lahore 54000, Pakistan.
Medicina (Kaunas). 2019 Sep 9;55(9):579. doi: 10.3390/medicina55090579.
: Dyslipidemia is gaining much attention among healthcare professionals because of its high association with the malfunctioning of a number of normal physiological and metabolic processes in the body. Obesity is directly interconnected with dyslipidemia and is said to be a denouement of hyperlipidemia and, if left untreated, may lead to intense damage to organs that are directly involved in fat metabolism. The objective of this study was to investigate the synergistic antiobesity and anti-hyperlipidemic activities along with hepato- and renoprotective potential of nanoemulsomes (NES) of lovastatin (LTN)-loaded ginger (GR) and garlic (GL) oils. : LTN nanoemulsomes co-encapsulated with GR oil and GL oil were prepared by a thin hydration technique. Eight-week-old male Wistar rats weighing 200-250 g were induced with hyperlipidemia via a high-fat diet (HFD) comprising 40% beef tallow. Body weight, serum biochemical lipid parameters, and those for liver and kidney functions, serum TC, LDL-C, vLDL-C, HDL-C, TG, atherogenic index (AI), ALT, AFT, ALP, γ-GT, total protein (TP), serum albumin and globulin ratio (A/G), serum creatinine, blood urea nitrogen (BUN) and blood urea, and histopathology of hematoxylin and eosin (H&E) stained liver and kidney sections of all aforementioned groups were examined in the treated animals. : Nanoemulsomes of LTN-loaded GR and GL oils provided synergistic effects with LTN, exerted better ameliorative actions in reducing serum TC, LDL-C, vLDL-C, triglycerides, and AI, and improved serum HDL-C levels. Serum ALT, AST, ALP, and γ-GT levels were in the normal range for nanoemulsome groups. H&E stained liver and kidney sections of these animals confirmed better hepatoprotective and renoprotective effects than LTN alone. Serum biochemical parameters for renal functions also claimed to be in the moderate range for nanoemulsome-treated groups. : This study demonstrated that nanoemulsomes of LTN-loaded GR and GL oils synergistically provided better antihyperlipidemic, hepatoprotective, and renoprotective effects as compared to LTN alone.
高脂血症由于与体内许多正常生理和代谢过程的功能障碍密切相关，因此引起了医疗保健专业人员的广泛关注。肥胖与高脂血症直接相关，被认为是高血脂的结果，如果不加以治疗，可能会导致直接参与脂肪代谢的器官受到严重损害。本研究旨在探讨载洛伐他汀（LTN）的姜（GR）和大蒜（GL）油纳米乳剂（NES）的协同抗肥胖和抗高脂血症活性以及肝和肾保护潜力。通过薄水技术制备载洛伐他汀（LTN）的纳米乳剂，同时包封姜（GR）油和大蒜（GL）油。将 200-250g 的 8 周龄雄性 Wistar 大鼠用高脂饮食（HFD）诱导高脂血症，HFD 由 40%的牛脂组成。在治疗动物中检查了体重、血清生化脂质参数以及肝功能和肾功能、血清 TC、LDL-C、VLDL-C、HDL-C、TG、致动脉粥样硬化指数（AI）、ALT、AFT、ALP、γ-GT、总蛋白（TP）、血清白蛋白和球蛋白比值（A/G）、血清肌酐、血尿素氮（BUN）和血尿素、以及苏木精和伊红（H&E）染色的肝和肾组织切片。结果显示，载洛伐他汀的 GR 和 GL 油纳米乳剂与 LTN 具有协同作用，在降低血清 TC、LDL-C、VLDL-C、甘油三酯和 AI 方面表现出更好的改善作用，并提高了血清 HDL-C 水平。纳米乳剂组的血清 ALT、AST、ALP 和γ-GT 水平均在正常范围内。这些动物的 H&E 染色肝和肾组织切片证实了比 LTN 单独治疗更好的保肝和保肾作用。用于肾功能的血清生化参数也被认为处于纳米乳剂治疗组的适度范围内。本研究表明，与 LTN 单独治疗相比，载洛伐他汀的 GR 和 GL 油纳米乳剂具有协同作用，可提供更好的抗高脂血症、保肝和保肾作用。
