Zaïri Amira, Nouir Sahar, Zarrouk Amira, Haddad Houda, Khélifa Améni, Achour Lotfi
Laboratory of Biochemistry Faculty of Medicine Sousse Tunisia.
Laboraory BIOVAL High Institute of Biotechnology Monastir Tunisia.
Food Sci Nutr. 2020 Jul 19;8(9):4805-4813. doi: 10.1002/fsn3.1755. eCollection 2020 Sep.
Although bioactivities of (OE have been widely described, most of them were related to its methanolic extracts or its essential oils, While data related to aqueous extracts still very scarce. Thus, in this study, the phytochemical composition, the antioxidant activity, the cytotoxic potential, and the allelopathic potential of aqueous leaf extracts from two varieties of were investigated and compared. High-performance liquid chromatography (HPLC) was used to identify and quantify the constituents of the tested plants, and spectrophotometric methods to evaluate antioxidant activities. The cytotoxic potential was investigated using murine oligodendrocytes (158N) while germination seeds' test was used for allelopathic activity. HPLC analysis showed the presence of 10 phenolic compounds in both extracts. Chemlali variety showed the highest antioxidant and allelopathic activities. Regarding the cytotoxicity effect, a significant increase in cell viability was observed with both of our extracts compared to untreated cells. These results confirm that aqueous extracts from OE produce a range of substances with potential antioxidant, antifungal, and allelopathic effects without toxic effects. Thus, they could be used as an alternative of chemical compounds.
尽管油橄榄叶（OE）的生物活性已被广泛描述，但其大多数生物活性都与其甲醇提取物或精油有关，而关于水提取物的数据仍然非常稀少。因此，在本研究中，对两个油橄榄品种叶水提取物的植物化学成分、抗氧化活性、细胞毒性潜力和化感潜力进行了研究和比较。采用高效液相色谱法（HPLC）对受试植物的成分进行鉴定和定量，并采用分光光度法评估抗氧化活性。利用小鼠少突胶质细胞（158N）研究细胞毒性潜力，同时采用发芽种子试验评估化感活性。HPLC分析表明，两种提取物中均存在10种酚类化合物。Chemali品种表现出最高的抗氧化和化感活性。关于细胞毒性作用，与未处理的细胞相比，两种提取物均观察到细胞活力显著增加。这些结果证实，油橄榄叶水提取物可产生一系列具有潜在抗氧化、抗真菌和化感作用且无毒性作用的物质。因此，它们可作为化学化合物的替代品。
