Dianatdar Farzaneh, Etemadifar Zahra
Department of Cell and Molecular Biology & Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, 8174673441, Iran.
World J Microbiol Biotechnol. 2025 Apr 28;41(5):149. doi: 10.1007/s11274-025-04371-1.
Antibiotic residues, as emerging micropollutants in surface water, threaten human health and ecosystems. The degradation of antibiotics is an essential process for safeguarding public health. This study identifies and optimizes a novel yeast strain for co-amoxiclav (AMC) degradation. Meyerozyma guilliermondii DF3 (Accession No. MZ477354) was isolated from soil using a basal mineral medium (BMM) supplemented with AMC. The strain degraded 75% of AMC within 72 h. Key culture conditions (temperature (20-35 °C), carbon sources (glucose/glycerol), inoculum size (1-5% v/v), and pH (5-9) were optimized via single-factor experiments and Box-Behnken design (BBD). Residual antibiotic levels were quantified by UV spectroscopy and HPLC. Optimal biodegradation occurred with glycerol/glucose, pH 6, and 1% inoculum (1 × 10⁸ cells/mL) at 30 °C. Response surface methodology (RSM) predicted complete degradation (100%) at pH 6.25, 1.02% inoculum, and 95.76 h, with pH being the most influential factor. These findings highlight M. guilliermondii DF3 as a promising candidate for AMC bioremediation in aquatic environments, advancing yeast-based strategies for water treatment.
抗生素残留作为地表水中新出现的微污染物，威胁着人类健康和生态系统。抗生素的降解是保障公众健康的一个重要过程。本研究鉴定并优化了一种用于降解阿莫西林克拉维酸钾（AMC）的新型酵母菌株。季也蒙毕赤酵母DF3（登录号MZ477354）是使用添加了AMC的基础矿物培养基（BMM）从土壤中分离得到的。该菌株在72小时内降解了75%的AMC。通过单因素实验和Box-Behnken设计（BBD）对关键培养条件（温度（20 - 35°C）、碳源（葡萄糖/甘油）、接种量（1 - 5% v/v）和pH值（5 - 9））进行了优化。残留抗生素水平通过紫外光谱法和高效液相色谱法进行定量。在30°C下，以甘油/葡萄糖为碳源、pH值为6、接种量为1%（1×10⁸个细胞/mL）时发生最佳生物降解。响应面法（RSM）预测在pH值为6.25、接种量为1.02%和95.76小时时可实现完全降解（100%），其中pH值是最具影响力的因素。这些发现突出了季也蒙毕赤酵母DF3作为水生环境中AMC生物修复的一个有前景的候选菌株，推动了基于酵母的水处理策略。
