Rosselle Léa, Cantelmo Anna Rita, Barras Alexandre, Skandrani Nadia, Pastore Michael, Aydin Duygu, Chambre Laura, Sanyal Rana, Sanyal Amitav, Boukherroub Rabah, Szunerits Sabine
Univ. Lille, CNRS, Centrale Lille, Yncréa ISEN, Univ. Polytechnique Hauts-de-France, UMR 8520 - IEMN, F-59000 Lille, France.
Biomater Sci. 2020 Nov 7;8(21):5911-5919. doi: 10.1039/d0bm01535k. Epub 2020 Sep 30.
A myriad of topical therapies and dressings are available to the clinicians for wound healing skin, but only a very few have shown their effectiveness in promoting wound repair due to challenges in controlling drug release. To address this issue, in this work, a near infrared (NIR)-light activable cryogel based on butyl methacrylate (BuMA) and poly(ethylene glycol) methyl ether methacrylate (PEGMEMA) incorporated with reduced graphene oxide (rGO) was fabricated. The obtained cryogel provides the required hydrophilicity beneficial for wound treatment. The excellent photo-thermal properties of rGO allow for heating the cryogel, which results in subsequent swelling of the cryogel (CG) followed by release of the encapsulated drug load, cefepime in our case. Without photothermal activation, no release of payload was observed. The potential of this bandage for wound healing was examined using an ex vivo human skin model infected with Staphylococcus aureus (S. aureus). Apart from the efficacy of the cryogel based wound healing system, our results also suggest that the ex vivo wound model evaluated here provides a rapid and valuable tool to study superficial skin infections in humans and test the efficacy of antimicrobial agents.
临床医生可获得多种用于伤口愈合皮肤的局部治疗方法和敷料，但由于药物释放控制方面的挑战，只有极少数方法在促进伤口修复方面显示出有效性。为了解决这个问题，在这项工作中，制备了一种基于甲基丙烯酸丁酯（BuMA）和聚（乙二醇）甲基丙烯酸甲酯（PEGMEMA）并掺入还原氧化石墨烯（rGO）的近红外（NIR）光可激活冷冻凝胶。所获得的冷冻凝胶提供了有利于伤口治疗的所需亲水性。rGO优异的光热性能能够加热冷冻凝胶，这导致冷冻凝胶（CG）随后膨胀，进而释放包封的药物负载，在我们的案例中是头孢吡肟。没有光热激活时，未观察到有效负载的释放。使用感染金黄色葡萄球菌（S. aureus）的离体人体皮肤模型研究了这种绷带用于伤口愈合的潜力。除了基于冷冻凝胶的伤口愈合系统的功效外，我们的结果还表明，此处评估的离体伤口模型为研究人类浅表皮肤感染和测试抗菌剂的功效提供了一种快速且有价值的工具。
