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miR-194-5p 负调控兔骨骼肌卫星细胞的增殖和分化。

miR-194-5p negatively regulates the proliferation and differentiation of rabbit skeletal muscle satellite cells.

机构信息

Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, China.

Research Institute of Animal Husbandry of Ganzi Tibetan Autonomous Prefecture, Kangding, 626000, China.

出版信息

Mol Cell Biochem. 2021 Jan;476(1):425-433. doi: 10.1007/s11010-020-03918-0. Epub 2020 Sep 30.

DOI:10.1007/s11010-020-03918-0
PMID:32997306
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7867548/
Abstract

Skeletal muscle satellite cells (SMSCs), also known as a multipotential stem cell population, play a crucial role during muscle growth and regeneration. In recent years, numerous miRNAs have been associated with the proliferation and differentiation of SMSCs in a number of mammalian species; however, the regulatory mechanisms of miR-194-5p in rabbit SMSCs still remain scarce. In this study, miR-194-5p was first observed to be highly expressed in the rabbit leg muscle. Furthermore, both the mimics and inhibitor of miR-194-5p were used to explore its role in the proliferation and differentiation of rabbit SMSCs cultured in vitro. Results from both EdU and CCK8 assays showed that miR-194-5p inhibited the proliferation of SMSCs. Meanwhile, Mef2c was identified as a target gene of miR-194-5p based on the dual-luciferase reporter assay results. In addition, upregulation of miR-194-5p decreased the expression levels of Mef2c and MyoG during rabbit SMSCs differentiation on Days 3 and 7 of in vitro culture. Taken together, these data demonstrated that miR-194-5p negatively regulates the proliferation and differentiation of rabbit SMSCs by targeting Mef2c.

摘要

骨骼肌卫星细胞（SMSCs），也称为多能干细胞群体，在肌肉生长和再生过程中起着至关重要的作用。近年来，许多 miRNA 与多种哺乳动物物种中 SMSCs 的增殖和分化有关；然而，miR-194-5p 在兔 SMSCs 中的调控机制仍然很少。在这项研究中，首次观察到 miR-194-5p 在兔腿部肌肉中高度表达。此外，还使用 miR-194-5p 的模拟物和抑制剂来探索其在体外培养的兔 SMSCs 增殖和分化中的作用。EdU 和 CCK8 检测结果表明，miR-194-5p 抑制 SMSCs 的增殖。同时，根据双荧光素酶报告基因检测结果，确定 Mef2c 是 miR-194-5p 的靶基因。此外，上调 miR-194-5p 可降低兔 SMSCs 在体外培养第 3 天和第 7 天分化过程中 Mef2c 和 MyoG 的表达水平。综上所述，这些数据表明，miR-194-5p 通过靶向 Mef2c 负调控兔 SMSCs 的增殖和分化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4a9/7867548/078557aa9758/11010_2020_3918_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4a9/7867548/359d412dc49f/11010_2020_3918_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4a9/7867548/7314a6501d91/11010_2020_3918_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4a9/7867548/e36e482a298b/11010_2020_3918_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4a9/7867548/411db3e02d2e/11010_2020_3918_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4a9/7867548/4c9c321a6847/11010_2020_3918_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4a9/7867548/078557aa9758/11010_2020_3918_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4a9/7867548/359d412dc49f/11010_2020_3918_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4a9/7867548/7314a6501d91/11010_2020_3918_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4a9/7867548/e36e482a298b/11010_2020_3918_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4a9/7867548/411db3e02d2e/11010_2020_3918_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4a9/7867548/4c9c321a6847/11010_2020_3918_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4a9/7867548/078557aa9758/11010_2020_3918_Fig6_HTML.jpg

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