Department of Ecosystem and Public Health, Faculty of Veterinary Medicine, University of Calgary, Health Research Innovation Center 2C53, 3330 Hospital Drive NW, Calgary, AB T2N 4N1, Canada.
Poultry Health Services, 1-4 East Lake Ave NE, Airdrie, AB T4A 2G8, Canada.
Viruses. 2020 Sep 28;12(10):1096. doi: 10.3390/v12101096.
In this study, we aimed to molecularly characterize 14 whole genome sequences of chicken astrovirus (CAstV) isolated from samples obtained from white chick syndrome (WCS) outbreaks in Western Canada during the period of 2014-2019. Genome sequence comparisons showed all these sequences correspond to the novel Biv group from which no confirmed representatives were published in GenBank. Molecular recombination analyses using recombination detection software (i.e., RDP5 and SimPlot) and phylogenetic analyses suggest multiple past recombination events in open reading frame (ORF)1a, ORF1b, and ORF2. Our findings suggest that recombination events and the accumulation of point mutations may have contributed to the substantial genetic variation observed in CAstV and evidenced by the current seven antigenic sub-clusters hitherto described. This is the first paper that describes recombination events in CAstV following analysis of complete CAstV sequences originated in Canada.
在这项研究中，我们旨在对从 2014 年至 2019 年期间在加拿大西部爆发的白痢综合征（WCS）中采集的样本中分离出的 14 株鸡星状病毒（CAstV）的全基因组序列进行分子特征分析。基因组序列比较表明，所有这些序列均与新型 Biv 组相对应，而 GenBank 中尚未发表该组的确认代表序列。使用重组检测软件（即 RDP5 和 SimPlot）和系统发育分析进行的分子重组分析表明，ORF1a、ORF1b 和 ORF2 中存在多次过去的重组事件。我们的研究结果表明，重组事件和点突变的积累可能导致了 CAstV 中观察到的大量遗传变异，这一点从迄今为止描述的七个抗原亚群中得到了证实。这是第一篇在分析源自加拿大的完整 CAstV 序列后，描述 CAstV 中重组事件的论文。
