Chen Libao, Chen Ligong, Wang Xuejing, Huo Shuying, Li Yurong
College of Veterinary Medicine, Hebei Agricultural University, Veterinary Biological Technology Innovation Center of Hebei Province, Baoding 071001, China.
Institute of Animal Husbandry and Veterinary Medicine of Hebei Province, Baoding 071000, China.
Animals (Basel). 2022 Oct 21;12(20):2873. doi: 10.3390/ani12202873.
Enteric viruses, as a potential pathogen, have been found to be vital causes of economic losses in poultry industry worldwide. The enteric viruses widely studied to date mainly include avian nephritis virus (ANV), avian reovirus (ARe), chicken astrovirus (CAstV), chicken parvovirus (ChPV), fowl adenovirus group I (FAdV-1), infectious bronchitis virus (IBV), and avian rotavirus (ARoV). This paper aimed to identify single and multiple infections of the seven enteric viruses using the data obtained from positive 145 enteric virus samples in poultry flocks from different areas in Hebei Province, throughout the period from 2019 to 2021. Next, the correlation between bird age and clinical signs was investigated using PCR and RT-PCR techniques. Furthermore, the whole genomes of seven parvovirus strains and open reading frame 2 (ORF2) of six CAstV strains and eight ANV strains were sequenced for phylogenetic analysis and recombination analysis, to characterize the viruses and evaluate species correlation and geographic patterns. A total of 11 profiles of virus combinations were detected; 191 viruses were detected in 145 samples; 106 single infections were reported in 73.1% of the samples; and multiple infections were detected in the remaining 26.9%. For viruses, 69% of ChPV was correlated with single infection, while ANV (61.4%) and CAstV (56.1%) were correlated with multiple infections. However, IBV and ARe were not detected in any of the samples. Recombination events were reported in parvovirus, and all CAstV sequences investigated in this paper were included within genotype Bii. The eight ANV strains pertained to different subtypes with significant differences. The above results revealed for the first time the complexity of enteric viruses over the past several years, thus contributing to disease prevention and control in the future.
肠道病毒作为一种潜在病原体，已被发现是全球家禽业经济损失的重要原因。迄今为止广泛研究的肠道病毒主要包括禽肾炎病毒（ANV）、禽呼肠孤病毒（ARe）、鸡星状病毒（CAstV）、鸡细小病毒（ChPV）、I群禽腺病毒（FAdV-1）、传染性支气管炎病毒（IBV）和禽轮状病毒（ARoV）。本文旨在利用2019年至2021年期间从河北省不同地区家禽群中145份肠道病毒阳性样本获得的数据，鉴定这七种肠道病毒的单一感染和多重感染情况。接下来，使用PCR和RT-PCR技术研究鸡龄与临床症状之间的相关性。此外，对七个细小病毒株的全基因组以及六个CAstV株和八个ANV株的开放阅读框2（ORF2）进行测序，用于系统发育分析和重组分析，以鉴定病毒并评估种属相关性和地理分布模式。共检测到11种病毒组合谱；在145个样本中检测到191种病毒；73.1%的样本报告有106次单一感染；其余26.9%检测到多重感染。对于病毒而言,69%的ChPV与单一感染相关，而ANV（61.4%）和CAstV（56.1%）与多重感染相关。然而，在任何样本中均未检测到IBV和ARe。细小病毒中报告有重组事件，本文研究的所有CAstV序列均属于Bii基因型。八个ANV株属于不同亚型，存在显著差异。上述结果首次揭示了过去几年肠道病毒的复杂性，从而有助于未来的疾病预防和控制。
