Departamento de Ciências da Natureza, Campus Professora Cinobelina Elvas, Universidade Federal do Piauí, Bom Jesus, PI, Brasil.
Laboratório de Desenvolvimento e Ensaios de Medicamentos, Centro de Ciências Biológicas e da Saúde, Universidade Estadual da Paraíba, Campina Grande, PB, Brasil.
Nat Prod Res. 2020 Dec;34(23):3382-3387. doi: 10.1080/14786419.2019.1566724. Epub 2019 Jan 28.
In this study, the chemical composition, genotoxic, cytotoxic and antibacterial-modulating activities of the (NPpE) extract was evaluated. The fingerprint chromatogram was determined using HPLC-DAD. The NPpE Minimal Inhibitory Concentration (MIC), as well as that of antimicrobial drugs in the presence and absence of the extract, were evaluated using the microdilution method against Gram positive bacteria. assays with mice were used for the determination of the extract's genotoxicity and cytotoxicity. The presence of the polyphenol catechin was confirmed in the extract. The extract showed significant antimicrobial activity (MIC ≤ 1000 µg mL) against and . When the NPpE was associated with several antimicrobials, the MIC of most of these were significantly reduced (P < 0.001) demonstrating good prospective usage in antimicrobial therapy. The extract has mutagenic and cytotoxic potential, however, further studies should be performed to confirm their toxicity.
在这项研究中，评估了 （NPpE）提取物的化学成分、遗传毒性、细胞毒性和抗菌调节活性。采用 HPLC-DAD 法测定指纹图谱。采用微量稀释法测定革兰氏阳性菌的最小抑菌浓度（MIC）以及提取物存在和不存在时抗菌药物的 MIC。使用小鼠实验测定提取物的遗传毒性和细胞毒性。提取物中含有多酚儿茶素。该提取物对 和 具有显著的抗菌活性（MIC≤1000µg mL）。当 NPpE 与几种抗菌药物联合使用时，大多数抗菌药物的 MIC 值显著降低（P<0.001），表明其在抗菌治疗中有很好的应用前景。该提取物具有致突变和细胞毒性潜力，但应进一步研究以确认其毒性。
