苜蓿花叶病毒的生物学和分子变异性对利雅得地区苜蓿作物的影响。

Biological and Molecular Variability of Alfalfa mosaic virus Affecting Alfalfa Crop in Riyadh Region.

机构信息

Plant Protection Department, College of Food and Agriculture Sciences, King Saud University, Kingdom of Saudi Arabia. Box 2460, Riyadh 11451.

Plant Protection Department, College of Food and Agriculture Sciences, King Saud University, Kingdom of Saudi Arabia. Box 2460, Riyadh 11451 ; Viruses and Phytoplasma Research Department, Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt.

出版信息

Plant Pathol J. 2013 Dec;29(4):410-7. doi: 10.5423/PPJ.OA.05.2013.0050.

DOI:10.5423/PPJ.OA.05.2013.0050

PMID:25288969

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4174816/

Abstract

In 2011-2012, sixty nine samples were collected from alfalfa plants showing viral infection symptoms in Riyadh region. Mechanical inoculation with sap prepared from two collected samples out of twenty five possitive for Alfalfa mosaic virus (AMV) by ELISA were produced systemic mosaic on Vigna unguiculata and Nicotiana tabacum, local lesion on Chenopodium amaranticolor and C. quinoa. Vicia faba indicator plants that induce mosaic and mottle with AMV-Sagir isolate and no infection with AMV-Wadi aldawasser isolate. Approximately 700-bp was formed by RT-PCR using AMV coat protein specific primer. Samples from infected alfalfa gave positive results, while healthy plant gave negative result using dot blot hybridization assay. The nucleotide sequences of the Saudi isolates were compared with corresponding viral nucleotide sequences reported in GenBank. The obtained results showed that the AMV from Australia, Brazil, Puglia and China had the highest similarity with AMV-Sajer isolate. While, the AMV from Spain and New Zealaland had the lowest similarity with AMV-Sajer and Wadi aldawasser isolates. The data obtained in this study has been deposited in the GenBank under the accession numbers KC434083 and KC434084 for AMV-Sajer and AMV- Wadialdawasser respectively. This is the first report regarding the gnetic make up of AMV in Saudi Arabia.

摘要

2011-2012 年，在利雅得地区采集了 69 个表现病毒感染症状的紫花苜蓿植物样本。用 25 个 ELISA 检测呈阳性的苜蓿花叶病毒（AMV）样本中的 2 个样本汁液进行机械接种，导致豇豆和烟草出现系统性花叶，苋菜和藜出现局部坏死斑。用 AMV-Sagir 分离物接种的蚕豆指示植物会出现花叶和斑驳症状，而用 AMV-Wadi aldawasser 分离物接种则没有感染。用 AMV 外壳蛋白特异性引物进行 RT-PCR 可形成约 700-bp 的产物。感染的紫花苜蓿样本呈阳性反应，而健康植物样本的斑点杂交检测呈阴性。与 GenBank 中报告的相应病毒核苷酸序列进行比较，沙特分离株的核苷酸序列。结果表明，来自澳大利亚、巴西、普利亚和中国的 AMV 与 AMV-Sajer 分离株具有最高的相似性。而来自西班牙和新西兰的 AMV 与 AMV-Sajer 和 Wadi aldawasser 分离株的相似性最低。本研究获得的数据已在 GenBank 中注册，登录号分别为 KC434083 和 KC434084，用于 AMV-Sajer 和 AMV-Wadialdawasser。这是沙特阿拉伯首次报道 AMV 的遗传构成。