Department of Plastic Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
Department of Radiation Oncology, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
Mol Med Rep. 2020 Nov;22(5):4236-4242. doi: 10.3892/mmr.2020.11509. Epub 2020 Sep 14.
DEAD‑Box Helicase 46 (DDX46) is an ATP‑dependent RNA helicase that plays a central role in transcription splicing and ribosome assembly. However, the role of DDX46 in cutaneous squamous cell carcinoma (CSCC) remains to be elucidated. The aim of the present study was to investigate the role of DDX46 in CSCC by assessing DDX46 expression levels in CSCC tissues and cell lines. The effect of DDX46 silencing on CSCC cell proliferation, apoptosis and autophagy were also analyzed. It was demonstrated that DDX46 was significantly overexpressed in CSCC tissues and cells (P<0.05). Furthermore, it was found that DDX46 silencing could dramatically inhibit cell proliferation (P<0.05). Moreover, cell apoptosis and autophagy were activated in DDX46 silencing groups (P<0.05). Therefore, the present results suggested that DDX46 was overexpressed in CSCC and that DDX46 silencing can inhibit cell proliferation by inducing apoptosis and activating autophagy. Thus, DDX46 may serve as a novel potential therapeutic target for CSCC.
DEAD-Box Helicase 46（DDX46）是一种 ATP 依赖性 RNA 解旋酶，在转录剪接和核糖体组装中发挥核心作用。然而，DDX46 在皮肤鳞状细胞癌（CSCC）中的作用仍有待阐明。本研究旨在通过评估 CSCC 组织和细胞系中 DDX46 的表达水平，研究 DDX46 在 CSCC 中的作用。还分析了 DDX46 沉默对 CSCC 细胞增殖、凋亡和自噬的影响。结果表明，DDX46 在 CSCC 组织和细胞中显著过表达（P<0.05）。此外，发现 DDX46 沉默可显著抑制细胞增殖（P<0.05）。此外，DDX46 沉默组细胞凋亡和自噬被激活（P<0.05）。因此，本研究结果表明，DDX46 在 CSCC 中过表达，DDX46 沉默通过诱导细胞凋亡和激活自噬抑制细胞增殖。因此，DDX46 可能成为 CSCC 的一种新的潜在治疗靶点。
