Heck Christian, Torchinsky Dmitry, Nifker Gil, Gularek Felix, Michaeli Yael, Weinhold Elmar, Ebenstein Yuval
School of Chemistry/Center for Nanoscience and Nanotechnology/Center for Light-Matter Interaction, Tel Aviv University, Ramat Aviv, Tel Aviv, Israel.
Institute of Organic Chemistry, RWTH Aachen University, D-52056 Aachen, Germany.
Nanoscale. 2020 Oct 15;12(39):20287-20291. doi: 10.1039/d0nr03694c.
Non-DNA labels are key components for the construction of functional DNA nanostructures. Here, we present a method to graft covalent labels onto DNA origami nanostructures in an enzymatic one-pot reaction. The DNA methyltransferase M.TaqI labels the DNA nanostructures with azide groups, which serve as universal attachment points via click chemistry. Direct labeling with fluorescent dyes is also demonstrated. The procedure yields structures with high fluorescence intensities and narrow intensity distributions. In combination with UV crosslinking it enables the creation of temperature-stable, intense fluorescent beacons.
非DNA标签是构建功能性DNA纳米结构的关键组件。在此，我们提出了一种在酶促一锅反应中将共价标签嫁接到DNA折纸纳米结构上的方法。DNA甲基转移酶M.TaqI用叠氮基团标记DNA纳米结构，这些叠氮基团通过点击化学作为通用连接点。还展示了用荧光染料直接标记。该方法产生具有高荧光强度和窄强度分布的结构。与紫外线交联相结合，它能够创建温度稳定的强荧光信标。
