State Key Laboratory of Microbial Technology, Shandong University, Binhai Road 72, Qingdao, 266237, Shandong, People's Republic of China.
National Glycoengineering Research Center, Shandong University, Binhai Road 72, Qingdao, 266237, Shandong, People's Republic of China.
Appl Biochem Biotechnol. 2021 Feb;193(2):377-388. doi: 10.1007/s12010-020-03433-4. Epub 2020 Oct 2.
One of the reasons hindering large-scale application of sophorolipids (SLs) is high production cost. In this study, six recombinant strains of Starmerella bombicola, sbEG1, sbEG2, sbCBH1, sbCBH1-2, sbBGL1, and sbCBH2 expressing cellulase genes eg1, eg2, cbh, cbh1-2, bgl1, and cbh2 from Penicillium oxalicum were respectively constructed. Four strains showed cellulase activities and were co-cultivated in fermentation media containing 2% glucose, 1% Regenerated Amorphous Cellulose (RAC), 2% glucose, and 1% RAC, respectively. After 7 days' cultivation, concentration of SLs in medium with 1% RAC (g/L) reached 1.879 g/L. When 2% glucose and 1% of RAC were both contained, the titer of SLs increased by 39.5% than that of control strain and increased by 68.8% than that in the medium with only 2% glucose. Results demonstrated that cellulase genes from filamentous fungi in S. bombicola can function to degrade lignocellulosic cellulose to produce SLs.
阻碍槐烷糖脂（SLs）大规模应用的原因之一是生产成本高。本研究构建了表达来自草酸青霉的纤维素酶基因 eg1、eg2、cbh、cbh1-2、bgl1 和 cbh2 的六株重组假丝酵母 Starmerella bombicola 菌株 sbEG1、sbEG2、sbCBH1、sbCBH1-2、sbBGL1 和 sbCBH2。四种菌株表现出纤维素酶活性，并分别在含有 2%葡萄糖、1%再生无定形纤维素（RAC）、2%葡萄糖和 1%RAC 的发酵培养基中进行共培养。培养 7 天后，含 1%RAC（g/L）的培养基中 SLs 的浓度达到 1.879 g/L。当含有 2%葡萄糖和 1%RAC 时，SLs 的产量比对照菌株提高了 39.5%，比仅含 2%葡萄糖的培养基提高了 68.8%。结果表明，假丝酵母中的丝状真菌纤维素酶基因可降解木质纤维素纤维素生产 SLs。
