Biochemistry and Biophysics, USA.
Biomedical Engineering, USA.
Biosens Bioelectron. 2020 Dec 1;169:112643. doi: 10.1016/j.bios.2020.112643. Epub 2020 Sep 21.
Detection of antibodies to upper respiratory pathogens is critical to surveillance, assessment of the immune status of individuals, vaccine development, and basic biology. The urgent need for antibody detection tools has proven particularly acute in the COVID-19 era. We report a multiplex label-free antigen microarray on the Arrayed Imaging Reflectometry (AIR) platform for detection of antibodies to SARS-CoV-2, SARS-CoV-1, MERS, three circulating coronavirus strains (HKU1, 229E, OC43) and three strains of influenza. We find that the array is readily able to distinguish uninfected from convalescent COVID-19 subjects, and provides quantitative information about total Ig, as well as IgG- and IgM-specific responses.
对上呼吸道病原体的抗体检测对于监测、评估个体的免疫状态、疫苗开发和基础生物学都至关重要。在 COVID-19 时代，对抗体检测工具的迫切需求已被证明尤为突出。我们报告了一种基于 Arrayed Imaging Reflectometry（AIR）平台的多重无标记抗原微阵列，用于检测 SARS-CoV-2、SARS-CoV-1、MERS、三种循环冠状病毒株（HKU1、229E、OC43）和三种流感病毒株的抗体。我们发现，该阵列能够轻松地区分未感染和康复的 COVID-19 患者，并提供有关总 Ig 以及 IgG 和 IgM 特异性反应的定量信息。
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