种子提取物对乳腺癌（MCF7）的抗增殖作用。
ANTIPROLIFERATIVE EFFECT ON BREAST CANCER (MCF7) OF SEED EXTRACTS.
作者信息
Adebayo Ismail Abiola, Arsad Hasni, Samian Mohd Razip
机构信息
Advanced Medical and Dental Institute, Universiti Sains Malaysia, 13200, Bertam, Pulau Pinang, Malaysia.
School of Biological Sciences, Universiti Sains Malaysia, 11800 USM, Pulau Pinang, Malaysia.
出版信息
Afr J Tradit Complement Altern Med. 2017 Jan 13;14(2):282-287. doi: 10.21010/ajtcam.v14i2.30. eCollection 2017.
BACKGROUND
belongs to plant family, Moringaceae and popularly called "wonderful tree", for it is used traditionally to cure many diseases including cancer in Africa and Asia, however, there is limited knowledge on cytotoxic activity of seeds on MCF7 breast cancer cell. The present study evaluated antiproliferative effect on MCF7 of the seed.
MATERIALS AND METHODS
Seeds of were grinded to powder and its phytochemicals were extracted using water and 80% ethanol solvents, part of the ethanolic extract were sequentially partitioned to fractions with four solvents (hexane, dichloromethane, chloroform, and n-butanol). Antiproliferative effects on MCF7 of the samples were determined. Finally, potent samples that significantly inhibited MCF7 growth were tested on MCF 10A.
RESULTS
Crude water extract, hexane and dichloromethane fractions of the seeds inhibited the proliferation of MCF7 with the following IC values 280 μg/ml, 130 μg/ml and 26 μg/ml respectively, however, of the 3 samples, only hexane fraction had minimal cytotoxic effect on MCF 10A (IC > 400μg/ml).
CONCLUSION
seed has antiproliferative effect on MCF7.
背景
属于辣木科植物，俗称“神奇之树”，因为在非洲和亚洲传统上它被用于治疗包括癌症在内的多种疾病，然而，关于其种子对MCF7乳腺癌细胞的细胞毒性活性的了解有限。本研究评估了该种子对MCF7的抗增殖作用。
材料与方法
将该植物的种子研磨成粉末，使用水和80%乙醇溶剂提取其植物化学成分，并将部分乙醇提取物依次用四种溶剂（己烷、二氯甲烷、氯仿和正丁醇）进行分配，得到不同的馏分。测定了各样本对MCF7的抗增殖作用。最后，对显著抑制MCF7生长的有效样本在MCF 10A细胞上进行了测试。
结果
种子的粗水提取物、己烷和二氯甲烷馏分抑制了MCF7的增殖，其IC值分别为280μg/ml、130μg/ml和26μg/ml，然而，在这3个样本中，只有己烷馏分对MCF 10A的细胞毒性最小（IC>400μg/ml）。
结论
该植物种子对MCF7有抗增殖作用。
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