IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, Bellaterra, 08193, Spain.
Consell Comarcal del Baix Llobregat, Servei de Control de Mosquits, Barcelona, Spain.
Emerg Microbes Infect. 2020 Dec;9(1):2236-2244. doi: 10.1080/22221751.2020.1830718.
mosquitoes have been experimentally demonstrated to be a competent vector for Zika virus (ZIKV) in different countries, but there are still some gaps related to the importance of in ZIKV transmission. Recent studies on Spanish populations showed controversial results for ZIKV transmission and no studies have been performed yet to detect infectious ZIKV in saliva of progeny of infected female mosquitoes. Herein, the horizontal transmission (HT) and vertical transmission (VT) of ZIKV in field-collected mosquitoes from Spain were evaluated for ZIKV strains (African I and Asian lineages) to better estimate the risk of ZIKV transmission by . The two field-collected populations assayed were infected by all tested ZIKV strains, however differences in terms of vector competence were detected depending on strain-population combination. Moreover, a higher susceptibility to the African I lineage strain than to the Asian lineage strain was observed in both mosquito populations. On the other hand, VT was demonstrated for both ZIKV lineages, detecting the virus in both males and females of the progeny of infected females, although importantly ZIKV dissemination and transmission were not detected in the infected females from the offspring. The results of the present study demonstrate that Spanish populations could sustain virus transmission in case of ZIKV introduction, but VT would play a poor role in the ZIKV epidemiology. Overall, our results provide helpful information to health authorities to establish efficient surveillance and vector control programmes for ZIKV.
蚊子已被实验证明是不同国家 Zika 病毒（ZIKV）的有效传播媒介，但与 ZIKV 传播的重要性相关仍存在一些空白。最近针对西班牙 种群的研究对 ZIKV 的传播结果存在争议，且尚未有研究检测到感染雌性蚊子后代唾液中的传染性 ZIKV。在此，评估了来自西班牙的野外采集的 蚊子对 ZIKV 株（非洲 I 谱系和亚洲谱系）的水平传播（HT）和垂直传播（VT），以更好地估计 通过 传播 ZIKV 的风险。测试的两种野外采集的 种群均被所有测试的 ZIKV 株感染，但根据株-种群组合，发现了媒介效能的差异。此外，在两个蚊子种群中，均观察到对非洲 I 谱系株的敏感性高于对亚洲谱系株的敏感性。另一方面，两种 ZIKV 谱系均证明了 VT，在感染雌性后代的雄性和雌性中均检测到了该病毒，但重要的是，未在感染雌性的后代中检测到 ZIKV 的传播。本研究的结果表明，西班牙 种群在引入 ZIKV 的情况下可能会维持病毒传播，但 VT 在 ZIKV 流行病学中作用不大。总的来说，我们的结果为卫生当局提供了有帮助的信息，以建立有效的 Zika 病毒监测和病媒控制计划。
