Chowdhury Ankan Dutta, Nasrin Fahmida, Gangopadhyay Rupali, Ganganboina Akhilesh Babu, Takemura Kenshin, Kozaki Ikko, Honda Hiroyuki, Hara Toshimi, Abe Fuyuki, Park Sungjo, Suzuki Tetsuro, Park Enoch Y
Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya Suruga-ku, Shizuoka 422-8529, Japan.
Department of Bioscience, Graduate School of Science and Technology, Shizuoka University, 836 Ohya Suruga-ku, Shizuoka 422-8529, Japan.
Biosens Bioelectron. 2020 Dec 15;170:112657. doi: 10.1016/j.bios.2020.112657. Epub 2020 Sep 26.
In this report, we have examined the distance- and size-dependent localized surface plasmon resonance (LSPR) between fluorescent quantum dots (QDs) and adjacent gold nanoparticles (AuNPs) to provide a comprehensive evaluation, aiming for practical application in biosensing platform. A series of peptides with different chain lengths, connected between QDs and AuNPs is initially applied to prepare various CdSe QDs-peptide-AuNP systems to optimize LSPR signal. Separation distance between two nanoparticles of these systems before and after conjugation is also confirmed by quantum mechanical modeling and corroborated with their LSPR influenced fluorescence variations. After detailed optimizations, it can be noted that larger sized AuNPs make strong quenching of QDs, which gradually shows enhancement of fluorescence with the increment of distance and the smaller sized AuNPs. Depending on the requirement, it is possible to tune the optimized structure of the CdSe QD-peptide-AuNP nanostructures for the application. In this work, two different structural designs with different peptide chain length are chosen to construct two biosensor systems, observing their fluorescence enhancement and quenching effects, respectively. Using different structural orientation of these biosensors, two nanoconjugates has applied for detection of norovirus and influenza virus, respectively to confirm their application in sensing.
在本报告中，我们研究了荧光量子点（QDs）与相邻金纳米颗粒（AuNPs）之间距离和尺寸依赖性的局域表面等离子体共振（LSPR），以进行全面评估，目标是在生物传感平台中实现实际应用。最初应用一系列连接在量子点和金纳米颗粒之间、具有不同链长的肽来制备各种CdSe量子点-肽-金纳米颗粒系统，以优化LSPR信号。这些系统中两个纳米颗粒在共轭前后的分离距离也通过量子力学建模得到证实，并与其受LSPR影响的荧光变化相印证。经过详细优化后，可以注意到较大尺寸的金纳米颗粒会使量子点强烈猝灭，随着距离增加和金纳米颗粒尺寸减小，荧光逐渐增强。根据需求，可以调整CdSe量子点-肽-金纳米颗粒纳米结构的优化结构以用于实际应用。在这项工作中，选择两种具有不同肽链长度的不同结构设计来构建两个生物传感器系统，分别观察它们的荧光增强和猝灭效果。利用这些生物传感器的不同结构取向，两种纳米共轭物分别应用于检测诺如病毒和流感病毒，以证实它们在传感中的应用。
