Shanxi Key Laboratory of Chinese Medicine Encephalopathy, Shanxi University of Chinese Medicine, Taiyuan, Shanxi Province, China.
Bureau of agriculture and rural affairs of Qianan, Tangshan, Hebei Province, China.
Environ Sci Pollut Res Int. 2021 Feb;28(6):6811-6817. doi: 10.1007/s11356-020-11054-x. Epub 2020 Oct 3.
This research was designed to investigate lipid peroxidation of the kidney of turtle (Mauremys reevesii) caused by cadmium. Turtles were injected intraperitoneally with cadmium at the concentration of 0 (control), 7.5, 15, and 30 mg/kg, and 5 turtles were taken from each group after exposure for 1 week (1 w), 2 weeks (2 w), and 3 weeks (3 w). Superoxide dismutase (SOD) and catalase (CAT) activities as well as glutathione (GSH) and malonyldialdehyde (MDA) contents in the homogenate of kidney tissue were analyzed. The results demonstrated that a short time of low dose of cadmium could stimulate the increase of SOD activity in the kidney of turtles, but a long time of high dose of cadmium could induce the decrease of SOD activity in the kidney of turtles. Cadmium could decrease CAT activity and GSH content in turtle kidney, but increased MDA content in turtle kidney. There were some other effects on the turtles, such as depression and diarrhea. The experimental results indicate that cadmium causes temporary oxidative stress on the kidney of turtles.
本研究旨在探讨镉对龟（中华草龟）肾脏的脂质过氧化作用。龟经腹腔注射不同浓度（0 对照组，7.5、15、30mg/kg）的镉，每组 5 只，分别于暴露后 1 周（1w）、2 周（2w）和 3 周（3w）取肾组织匀浆，检测超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性以及谷胱甘肽（GSH）和丙二醛（MDA）含量。结果表明，短时间、低剂量镉可刺激龟肾 SOD 活性增加，但长时间、高剂量镉可诱导龟肾 SOD 活性降低。镉可降低龟肾 CAT 活性和 GSH 含量，增加 MDA 含量。此外，镉还对龟产生了其他影响，如抑郁和腹泻。实验结果表明，镉对龟的肾脏造成了短暂的氧化应激。
