Yamamoto Tatsuya, Sakakibara Ryuji, Uchiyama Tomoyuki, Kuwabara Satoshi
Department of Rehabilitation Sciences, Chiba Prefectural University of Health Sciences, Chiba, Japan.
Department of Neurology, Chiba University Graduate School of Medicine, Chiba, Japan.
Front Neurosci. 2020 Sep 3;14:917. doi: 10.3389/fnins.2020.00917. eCollection 2020.
The patients with Parkinson's disease (PD) present with lower urinary tract symptoms (LUTS), but the efficacy of subthalamic nucleus deep brain stimulation (STN-DBS) on LUTS is unknown. The medial prefrontal cortex (mPFC) is a known higher micturition center which are modulated by STN-DBS. We aim to clarify STN-DBS-related changes in the neuronal activity of the mPFC in terms of bladder contraction, using normal and PD rats.
Experiments in normal and 6-hydroxydopamine hemi-lesioned PD rats were conducted under urethane anesthesia. STN-DBS was applied to the left STN, with simultaneous monitoring of bladder contractions. The mPFC's local field potential (LFP) was recorded before, during, and after STN-DBS ( = 6: normal rats, = 6: PD rats). Before, during and after STN-DBS ( = 5: normal rats, = 6: PD rats), extracellular fluid was collected from mPFC.
STN-DBS significantly increased bladder inter-contraction interval. STN-DBS significantly decreased mPFC alpha power in normal rat and increased alpha power in PD rat. The mPFC levels of levodopa, dopamine, serotonin and their metabolites in PD rats decreased significantly during and after STN-DBS, whereas the serotonin and its metabolites and homovanillic acid (HVA) levels decreased significantly in normal rats following STN-DBS.
STN-DBS increased intercontraction intervals for the bladder in both normal and PD rats, as assessed by alpha power and catecholamine levels in mPFC, probably due to changes in neural activity. The effect of STN-DBS on mPFC levels of catecholamine differentiated between the normal and PD rats.
帕金森病（PD）患者存在下尿路症状（LUTS），但丘脑底核深部脑刺激（STN-DBS）对LUTS的疗效尚不清楚。内侧前额叶皮质（mPFC）是已知的高级排尿中枢，受STN-DBS调节。我们旨在使用正常大鼠和PD大鼠，阐明STN-DBS相关的mPFC神经元活动在膀胱收缩方面的变化。
在乌拉坦麻醉下对正常大鼠和6-羟基多巴胺半侧损伤的PD大鼠进行实验。将STN-DBS应用于左侧STN，同时监测膀胱收缩。在STN-DBS前、中、后记录mPFC的局部场电位（LFP）（正常大鼠n = 6，PD大鼠n = 6）。在STN-DBS前、中、后（正常大鼠n = 5，PD大鼠n = 6），从mPFC收集细胞外液。
STN-DBS显著增加膀胱收缩间期。STN-DBS显著降低正常大鼠的mPFCα功率，增加PD大鼠的α功率。PD大鼠在STN-DBS期间及之后，mPFC中的左旋多巴、多巴胺、5-羟色胺及其代谢产物水平显著降低，而正常大鼠在STN-DBS后5-羟色胺及其代谢产物和高香草酸（HVA）水平显著降低。
通过mPFC中的α功率和儿茶酚胺水平评估，STN-DBS增加了正常大鼠和PD大鼠膀胱的收缩间期，这可能是由于神经活动的变化。STN-DBS对mPFC儿茶酚胺水平的影响在正常大鼠和PD大鼠之间存在差异。
