Wang Youyou, Zhang Congying, Liang Jiao, Wu Lufang, Gao Wenbin, Jiang Jizhi
College of Life Science, Institute of Life Science and Green Development, Hebei University, Baoding, China.
Front Microbiol. 2020 Sep 9;11:536083. doi: 10.3389/fmicb.2020.536083. eCollection 2020.
Potato late blight, caused by (Mont.) de Bary, represents a great food security threat worldwide and is difficult to control. Recently, spp. have been considered biocontrol agents to control many plant diseases. Here, WL-2 was selected as a potent strain against mycelium growth, and its functional metabolite was identified as Iturin A via electrospray ionization mass spectrometry (ESI-MS). Analyses using scanning electron microscopy (SEM) and transmission electron microscopy (TEM) revealed that Iturin A caused cell membrane disruption and an irregular internal cell structure. In addition, Iturin A triggered oxidative stress reactions similarly to reactive oxygen species (ROS) in cells and caused mitochondrial damage, including mitochondrial membrane potential (MMP), mitochondrial respiratory chain complex activity (MRCCA), and ATP production decline. These results highlight that the cell structure disruption, oxidative stress, and energy supply dysfunction induced by Iturin A play an important role in inhibiting . Additionally, WL-2 and Iturin A have great potential for inhibiting mycelium growth and controlling potato late blight in the future.
由致病疫霉（Mont.）德巴里引起的马铃薯晚疫病，在全球范围内对粮食安全构成巨大威胁且难以控制。最近，芽孢杆菌属已被视为控制多种植物病害的生物防治剂。在此，WL - 2被选为抑制致病疫霉菌丝体生长的高效菌株，其功能代谢产物通过电喷雾电离质谱（ESI - MS）鉴定为伊枯草菌素A。使用扫描电子显微镜（SEM）和透射电子显微镜（TEM）进行的分析表明，伊枯草菌素A导致细胞膜破坏和细胞内部结构不规则。此外，伊枯草菌素A在致病疫霉细胞中引发了类似于活性氧（ROS）的氧化应激反应，并导致线粒体损伤，包括线粒体膜电位（MMP）、线粒体呼吸链复合体活性（MRCCA）和ATP产生下降。这些结果表明，伊枯草菌素A诱导的细胞结构破坏、氧化应激和能量供应功能障碍在抑制致病疫霉方面发挥着重要作用。此外，WL - 2和伊枯草菌素A在未来抑制致病疫霉菌丝体生长和控制马铃薯晚疫病方面具有巨大潜力。
