Perdiguero Pedro, Morel Esther, Tafalla Carolina
Fish Immunology and Pathology Group, Animal Health Research Center (CISA-INIA), Valdeolmos, 28130 Madrid, Spain.
Biology (Basel). 2021 Jun 9;10(6):511. doi: 10.3390/biology10060511.
Single-cell sequencing technologies capable of providing us with immune information from dozens to thousands of individual cells simultaneously have revolutionized the field of immunology these past years. However, to date, most of these novel technologies have not been broadly applied to non-model organisms such as teleost fish. In this study, we used the 10× Genomics single cell RNA sequencing technology and used it to analyze for the first time in teleost fish the transcriptional pattern of single B cells from peripheral blood. The analysis of the data obtained in rainbow trout revealed ten distinct cell clusters that seem to be associated with different subsets and/or maturation/differentiation stages of circulating B cells. The potential characteristics and functions of these different B cell subpopulations are discussed on the basis of their transcriptomic profile. The results obtained provide us with valuable information to understand the biology of teleost B cells and offer us a repertoire of potential markers that could be used in the future to differentiate trout B cell subsets.
近年来，能够同时为我们提供数十到数千个单个细胞免疫信息的单细胞测序技术彻底改变了免疫学领域。然而，迄今为止，这些新技术大多尚未广泛应用于非模式生物，如硬骨鱼。在本研究中，我们使用了10×基因组学单细胞RNA测序技术，并首次在硬骨鱼中分析外周血单个B细胞的转录模式。对虹鳟鱼获得的数据进行分析后发现了十个不同的细胞簇，这些细胞簇似乎与循环B细胞的不同亚群和/或成熟/分化阶段相关。基于它们的转录组概况，讨论了这些不同B细胞亚群的潜在特征和功能。所获得的结果为我们了解硬骨鱼B细胞生物学提供了有价值的信息，并为我们提供了一系列潜在的标记物，这些标记物未来可用于区分鳟鱼B细胞亚群。
