Williamson-Benavides Bruce A, Sharpe Richard M, Nelson Grant, Bodah Eliane T, Porter Lyndon D, Dhingra Amit
Molecular Plant Sciences, Washington State University, Pullman, WA, United States.
Department of Horticulture, Washington State University, Pullman, WA, United States.
Front Genet. 2020 Aug 18;11:950. doi: 10.3389/fgene.2020.00950. eCollection 2020.
(pea) is rapidly emerging as an inexpensive and significant contributor to the plant-derived protein market. Due to its nitrogen-fixation capability, short life cycle, and low water usage, pea is a useful cover-and-break crop that requires minimal external inputs. It is critical for sustainable agriculture and indispensable for future food security. Root rot in pea, caused by the fungal pathogen f. sp. (), can result in a 15-60% reduction in yield. It is urgent to understand the molecular basis of interaction in pea to develop root rot tolerant cultivars. A complementary genetics and gene expression approach was undertaken in this study to identify -responsive genes in four tolerant and four susceptible pea genotypes. Time course RNAseq was performed on both sets of genotypes after the challenge. Analysis of the transcriptome data resulted in the identification of 42,905 differentially expressed contigs (DECs). Interestingly, the vast majority of DECs were overexpressed in the susceptible genotypes at all sampling time points, rather than in the tolerant genotypes. Gene expression and GO enrichment analyses revealed genes coding for receptor-mediated endocytosis, sugar transporters, salicylic acid synthesis, and signaling, and cell death were overexpressed in the susceptible genotypes. In the tolerant genotypes, genes involved in exocytosis, and secretion by cell, the anthocyanin synthesis pathway, as well as the DRR230 gene, a pathogenesis-related (PR) gene, were overexpressed. The complementary genetic and RNAseq approach has yielded a set of potential genes that could be targeted for improved tolerance against root rot in . challenge produced a futile transcriptomic response in the susceptible genotypes. This type of response is hypothesized to be related to the speed at which the pathogen infestation advances in the susceptible genotypes and the preexisting level of disease-preparedness in the tolerant genotypes.
豌豆正迅速成为植物源蛋白质市场中一种价格低廉且重要的贡献者。由于其固氮能力、短生命周期和低用水量，豌豆是一种有用的覆盖和轮作作物，所需外部投入极少。它对可持续农业至关重要，对未来粮食安全不可或缺。由真菌病原体f. sp.（）引起的豌豆根腐病可导致产量降低15%至60%。迫切需要了解豌豆中相互作用的分子基础，以培育耐根腐病品种。本研究采用互补的遗传学和基因表达方法，在四种耐病和四种感病豌豆基因型中鉴定响应基因。在接种挑战后，对两组基因型都进行了时间进程RNA测序。转录组数据分析导致鉴定出42905个差异表达重叠群（DECs）。有趣的是，在所有采样时间点，绝大多数DECs在感病基因型中过度表达，而不是在耐病基因型中。基因表达和GO富集分析表明，编码受体介导的内吞作用、糖转运蛋白、水杨酸合成和信号传导以及细胞死亡的基因在感病基因型中过度表达。在耐病基因型中，参与胞吐作用、细胞分泌、花青素合成途径以及病程相关（PR）基因DRR230基因过度表达。互补的遗传学和RNA测序方法产生了一组潜在基因，可针对这些基因提高豌豆对根腐病的耐受性。接种挑战在感病基因型中产生了无效的转录组反应。据推测，这种类型的反应与病原体在感病基因型中的侵染速度以及耐病基因型中预先存在的抗病准备水平有关。
