Jernelv Ine L, Hjelme Dag Roar, Aksnes Astrid
Department of Electronic Systems, Norwegian University of Science and Technology (NTNU), O.S. Bragstads plass 2A, 7491 Trondheim, Norway.
Biomed Opt Express. 2020 Jun 18;11(7):3818-3829. doi: 10.1364/BOE.393617. eCollection 2020 Jul 1.
Fast and accurate continuous glucose monitoring is needed in future systems for control of blood glucose levels in type 1 diabetes patients. Direct spectroscopic measurement of glucose in the peritoneal cavity is an attractive alternative to conventional electrochemical sensors placed subcutaneously. We demonstrate the feasibility of fast glucose measurements in peritoneal fluid using a fibre-coupled tuneable mid-infrared quantum cascade laser. Mid-infrared spectra (1200-925 cm) of peritoneal fluid samples from pigs with physiological glucose levels (32-426 mg/dL, or 1.8-23.7 mmol/L) were acquired with a tuneable quantum cascade laser employing both transmission and attenuated total reflection (ATR) spectroscopy. Using partial least-squares regression, glucose concentrations were predicted with mean absolute percentage errors (MAPEs) of 8.7% and 12.2% in the transmission and ATR configurations, respectively. These results show that highly accurate concentration predictions are possible with mid-infrared spectroscopy of peritoneal fluid, and represent a first step towards a miniaturised optical sensor for intraperitoneal continuous glucose monitoring.
未来用于控制1型糖尿病患者血糖水平的系统需要快速且准确的连续血糖监测。对腹腔内葡萄糖进行直接光谱测量是皮下放置传统电化学传感器的一种有吸引力的替代方法。我们展示了使用光纤耦合可调谐中红外量子级联激光器在腹腔液中快速测量葡萄糖的可行性。使用可调谐量子级联激光器，采用透射光谱和衰减全反射（ATR）光谱，采集了生理血糖水平（32 - 426 mg/dL，即1.8 - 23.7 mmol/L）的猪腹腔液样本的中红外光谱（1200 - 925 cm）。使用偏最小二乘回归，在透射和ATR配置中预测葡萄糖浓度的平均绝对百分比误差（MAPE）分别为8.7%和12.2%。这些结果表明，通过腹腔液的中红外光谱可以实现高精度的浓度预测，这是迈向用于腹腔内连续血糖监测的小型光学传感器的第一步。
