利用荧光生物传感器研究太赫兹辐射对基因网络的影响。 （注：原文中“of by means of fluorescent biosensors”表述有误，正确的应该是“by means of fluorescent biosensors”，翻译时根据修正后的内容进行了翻译）
Study on the effects of terahertz radiation on gene networks of by means of fluorescent biosensors.
作者信息
Serdyukov Danil S, Goryachkovskaya Tatiana N, Mescheryakova Irina A, Bannikova Svetlana V, Kuznetsov Sergei A, Cherkasova Olga P, Popik Vasiliy M, Peltek Sergey E
机构信息
Laboratory of Molecular Biotechnologies of Federal Research Center Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences, 10 Lavrentiev Avenue, Novosibirsk 630090, Russia.
Kurchatov Genomics Center of Federal Research Center Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences, 10 Lavrentiev Avenue, Novosibirsk 630090, Russia.
出版信息
Biomed Opt Express. 2020 Aug 25;11(9):5258-5273. doi: 10.1364/BOE.400432. eCollection 2020 Sep 1.
Three novel fluorescent biosensors sensitive to terahertz (THz) radiation were developed via transformation of () cells with plasmids, in which a promotor of genes , , or controls the expression of a fluorescent protein. The biosensors were exposed to THz radiation from two sources: a high-intensity pulsed short-wave free electron laser and a low-intensity continuous long-wave IMPATT-diode-based device. The threshold and dynamics of fluorescence were found to depend on radiation parameters and exposure time. Heat shock or chemical stress yielded the absence of fluorescence induction. The biosensors are evaluated to be suitable for studying influence of THz radiation on the activity of gene networks related with considered gene promoters.
通过用质粒转化（）细胞，开发了三种对太赫兹（THz）辐射敏感的新型荧光生物传感器，其中基因、或的启动子控制荧光蛋白的表达。这些生物传感器暴露于来自两个源的太赫兹辐射：高强度脉冲短波自由电子激光器和低强度连续长波基于IMPATT二极管的装置。发现荧光的阈值和动力学取决于辐射参数和暴露时间。热休克或化学应激导致荧光诱导缺失。评估这些生物传感器适用于研究太赫兹辐射对与所考虑基因启动子相关的基因网络活性的影响。
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