Gruening Martina, Neuber Sven, Nestler Peter, Lehnfeld Jutta, Dubs Manuela, Fricke Katja, Schnabelrauch Matthias, Helm Christiane A, Müller Rainer, Staehlke Susanne, Nebe J Barbara
Department of Cell Biology, Rostock University Medical Center, Rostock, Germany.
Soft Matter and Biophysics, Institute of Physics, University of Greifswald, Greifswald, Germany.
Front Bioeng Biotechnol. 2020 Sep 8;8:1016. doi: 10.3389/fbioe.2020.01016. eCollection 2020.
Electrostatic forces at the cell interface affect the nature of cell adhesion and function; but there is still limited knowledge about the impact of positive or negative surface charges on cell-material interactions in regenerative medicine. Titanium surfaces with a variety of zeta potentials between -90 mV and +50 mV were generated by functionalizing them with amino polymers, extracellular matrix proteins/peptide motifs and polyelectrolyte multilayers. A significant enhancement of intracellular calcium mobilization was achieved on surfaces with a moderately positive (+1 to +10 mV) compared with a negative zeta potential (-90 to -3 mV). Dramatic losses of cell activity (membrane integrity, viability, proliferation, calcium mobilization) were observed on surfaces with a highly positive zeta potential (+50 mV). This systematic study indicates that cells do not prefer positive charges in general, merely moderately positive ones. The cell behavior of MG-63s could be correlated with the materials' zeta potential; but not with water contact angle or surface free energy. Our findings present new insights and provide an essential knowledge for future applications in dental and orthopedic surgery.
细胞界面处的静电力会影响细胞黏附的性质和功能；但在再生医学中，关于正表面电荷或负表面电荷对细胞与材料相互作用的影响，人们了解得仍然有限。通过用氨基聚合物、细胞外基质蛋白/肽基序和聚电解质多层膜对钛表面进行功能化处理，制备出了zeta电位在-90 mV至+50 mV之间的各种钛表面。与具有负zeta电位（-90至-3 mV）的表面相比，在具有适度正电位（+1至+10 mV）的表面上，细胞内钙动员显著增强。在具有高度正zeta电位（+50 mV）的表面上，观察到细胞活性（膜完整性、活力、增殖、钙动员）急剧丧失。这项系统性研究表明，细胞一般并不偏好正电荷，只是适度偏好正电荷。MG-63细胞的行为可能与材料的zeta电位相关；但与水接触角或表面自由能无关。我们的研究结果提供了新的见解，并为未来在牙科和矫形外科手术中的应用提供了重要知识。
