Staehlke Susanne, Springer Armin, Freitag Thomas, Brief Jakob, Nebe J Barbara
Department of Cell Biology, University Medical Center Rostock, D-18057 Rostock, Germany.
Medical Biology and Electron Microscopic Center, University Medical Center Rostock, D-18057 Rostock, Germany.
Dent J (Basel). 2020 Nov 10;8(4):127. doi: 10.3390/dj8040127.
The high biocompatibility, good mechanical properties, and perfect esthetics of ceramic dental materials motivate investigation into their suitability as an endosseous implant. Osseointegration at the interface between bone and implant surface, which is a criterion for dental implant success, is dependent on surface chemistry and topography. We found out earlier that osteoblasts on sharp-edged micro-topographies revealed an impaired cell phenotype and function and the cells attempted to phagocytize these spiky elevations in vitro. Therefore, micro-structured implants used in dental surgery should avoid any spiky topography on their surface. The sandblasted, acid-etched, and heat-treated yttria-stabilized zirconia (cer.face14) surface was characterized by scanning electron microscopy and energy dispersive X-ray. In vitro studies with human MG-63 osteoblasts focused on cell attachment and intracellular stress level. The cer.face 14 surface featured a landscape with nano-micro hills that was most sinusoidal-shaped. The mildly curved profile proved to be a suitable material for cell anchorage. MG-63 cells on cer.face 14 showed a very low reactive oxygen species (ROS) generation similar to that on the extracellular matrix protein collagen I (Col). Intracellular adenosine triphosphate (ATP) levels were comparable to Col. Ceramic cer.face 14, with its sinusoidal-shaped surface structure, facilitates cell anchorage and prevents cell stress.
陶瓷牙科材料具有高生物相容性、良好的机械性能和完美的美学效果，这促使人们对其作为骨内植入物的适用性进行研究。骨与种植体表面之间的骨结合是牙科种植成功的一个标准，它取决于表面化学和形貌。我们早些时候发现，在边缘尖锐的微观形貌上的成骨细胞显示出细胞表型和功能受损，并且细胞在体外试图吞噬这些尖刺状凸起。因此，牙科手术中使用的微结构种植体应避免其表面有任何尖刺状形貌。通过扫描电子显微镜和能量色散X射线对喷砂、酸蚀和热处理的氧化钇稳定氧化锆（cer.face14）表面进行了表征。对人MG-63成骨细胞的体外研究集中在细胞附着和细胞内应激水平上。cer.face 14表面具有纳米-微米级的起伏景观，大多呈正弦形状。这种轻微弯曲的轮廓被证明是一种适合细胞锚定的材料。cer.face 14上的MG-63细胞显示出非常低的活性氧（ROS）生成，类似于细胞外基质蛋白I型胶原（Col）上的情况。细胞内三磷酸腺苷（ATP）水平与Col相当。具有正弦形表面结构的陶瓷cer.face 14有助于细胞锚定并防止细胞应激。
