Patel H N, Vohra Y K, Singh R, Thomas V
Department of Biomedical Engineering, University of Alabama at Birmingham (UAB), Birmingham, AL, 35294, USA.
Center for Nanoscale Materials and Biointegration (CNMB), University of Alabama at Birmingham (UAB), Birmingham, AL, 35294, USA.
Mater Today Chem. 2020 Sep;17. doi: 10.1016/j.mtchem.2020.100323. Epub 2020 Aug 1.
Native extracellular matrix (ECM) possesses the biochemical cues to promote cell survival. However, decellularized, the ECM loses its cell supporting mechanical integrity. We report, here, a novel biohybrid vascular graft of polycaprolactone (PCL), poliglecaprone (PGC) incorporated with human biomatrix as functional materials for vascular tissue interfacing by electrospinning, thus harnessing the biochemical cues from the ECM and the mechanical integrity of the polymer blends. The fabricated fibro-porous tubular small diameter graft ( = 4 mm) from polymer blend was coated with a cocktail of collagenous matrix derived from human placenta called HuBiogel™. The compositional, morphological, and mechanical properties of graft were measured and compared with a non-coated tubular PCL/PGC graft using Fourier Transform infrared spectroscopy (FTIR), x-ray photoelectron spectroscopy (XPS), and scanning electron microscopy (SEM). BCA assay was used to calculate the protein content and coating-uniformity throughout the hybrid graft. Mechanical properties such as tensile strength (1.6 MPa), Young's modulus (2.4 MPa), burst pressure (>1900 mmHg), and suture retention strength (2.3 N) of hybrid graft were found to be comparable to native blood vessels. Protein coating has improved the hydrophilicity and the biocompatibility (cell viability and cell-attachment) enhanced with human umbilical vein endothelial cells (HUVECs) seeded onto the lumen layer of the graft over two weeks. The overall results promise this new biohybrid graft to be a potential candidate for vascular tissue interface and regeneration.
天然细胞外基质（ECM）具有促进细胞存活的生化信号。然而，脱细胞后，ECM失去了其支持细胞的机械完整性。我们在此报告一种新型的生物杂交血管移植物，它由聚己内酯（PCL）、聚乙交酯丙交酯（PGC）与人生物基质结合而成，作为功能性材料通过静电纺丝实现血管组织界面连接，从而利用来自ECM的生化信号以及聚合物共混物的机械完整性。由聚合物共混物制成的纤维多孔管状小直径移植物（内径 = 4 mm）涂覆有一种源自人胎盘的胶原基质混合物，称为HuBiogel™。使用傅里叶变换红外光谱（FTIR）、X射线光电子能谱（XPS）和扫描电子显微镜（SEM）对移植物的组成、形态和机械性能进行了测量，并与未涂覆的管状PCL/PGC移植物进行了比较。使用BCA测定法计算整个杂交移植物中的蛋白质含量和涂层均匀性。发现杂交移植物的机械性能，如拉伸强度（1.6 MPa）、杨氏模量（2.4 MPa）、爆破压力（>1900 mmHg）和缝线保留强度（2.3 N）与天然血管相当。蛋白质涂层提高了亲水性，并且在两周内将人脐静脉内皮细胞（HUVECs）接种到移植物的内腔层上后，生物相容性（细胞活力和细胞附着）得到增强。总体结果表明这种新型生物杂交移植物有望成为血管组织界面和再生的潜在候选物。
