Gupta Arvind Kumar, Kunnumbrath Arathi, Tayal Sakshi Garg, Mehan Anoushika, Sahay Rishabh, Kumar Utpal, Jeladharan Reshma, Anthony Michael Leonard, Singh Neha, Chandra Harish, Chowdhury Nilotpal
Department of Pathology & Laboratory Medicine, All India Institute of Medical Sciences, Rishikesh, India.
Scand J Clin Lab Invest. 2020 Dec;80(8):654-658. doi: 10.1080/00365513.2020.1827290. Epub 2020 Oct 5.
Estimates of Within-Subject and between subject biological variation for the white blood cell differential count (DC) have not been reported in South Asia. Therefore, we attempted to measure the short-term biological variation estimates for DC. The study was conducted on 28 healthy volunteers (15 males and 13 females). Blood from the volunteers was collected in the morning in K3-EDTA vials and analyzed in triplicate on the Sysmex XN-1000 analyzer, for six consecutive days. The Within subject, between subject and analytical coefficient of variation of the DC was calculated from the results by nested repeated measures ANOVA after outlier exclusion. The Reference change values (RCV) were also calculated. The within-subject variation for eosinophil Count and between subject variation for basophils in our study from South Asia was greater than the published European and American studies. Males and females showed similar biological variation for DC. The within-subject variation of other parameters (Neutrophils, Lymphocytes, Monocytes and Basophils) were similar or showed only mild differences to the published studies. The markedly different within-subject variation for Eosinophil counts suggest that the RCV for DC in South Asians need to be different from the published data in order to have clinical relevance. The Within-subject variation values of the other parameters seem transportable from the published European and American studies, but the small differences found mean that further regional estimates need to be reported for robust evidence of the same.
南亚地区尚未报告白细胞分类计数（DC）的受试者内和受试者间生物学变异估计值。因此，我们试图测量DC的短期生物学变异估计值。该研究对28名健康志愿者（15名男性和13名女性）进行。志愿者的血液于早晨采集到K3-乙二胺四乙酸（EDTA）试管中，并在Sysmex XN-1000分析仪上连续六天进行三次重复分析。在排除异常值后，通过嵌套重复测量方差分析从结果中计算DC的受试者内、受试者间和分析变异系数。还计算了参考变化值（RCV）。我们在南亚地区的研究中，嗜酸性粒细胞计数的受试者内变异和嗜碱性粒细胞的受试者间变异大于已发表的欧美研究。男性和女性在DC方面表现出相似的生物学变异。其他参数（中性粒细胞、淋巴细胞、单核细胞和嗜碱性粒细胞）的受试者内变异与已发表研究相似或仅显示出轻微差异。嗜酸性粒细胞计数的受试者内变异明显不同，这表明南亚人DC的RCV需要与已发表数据不同才能具有临床相关性。其他参数的受试者内变异值似乎可从已发表的欧美研究中移植，但发现的微小差异意味着需要报告进一步的区域估计值，以获得关于此的有力证据。
