Arkin C F, Medeiros L J, Pevzner L Z, Guertin B P, Kobos P J, Phelps J W, Smith S J
Am J Clin Pathol. 1987 May;87(5):628-32. doi: 10.1093/ajcp/87.5.628.
A quick, one and a half-minute qualitative microscopic scan was investigated as an alternative approach to the more labor-intensive 100-cell differential white blood cell count. The scanning results of 400 randomly selected hospital cases were compared with the on-line results of the 100-cell counts. Additionally, 50 cases selected to have a high percentage of abnormal results were each scanned and manually counted by four different readers. The results indicate that the scanning differential is equivalent to the 100-cell manual count in the detection of the presence of abnormal cell types such as immature granulocytes and blasts. Its ability to properly estimate the relative proportions of normal cells, especially lymphocytes, however, does not appear as reliable as the manual count. Most importantly, the analysis demonstrates that the scanning differential count exhibits a set of advantages and disadvantages that is complementary to those of the "three-part differential" technic provided by the newer generation automated hematologic analyzers. The authors therefore propose that these two procedures used in combination offer a suitable alternative to the manual 100-cell differential count.
研究了一种快速的、一分半钟的定性显微镜扫描方法，作为替代更为繁琐的100个白细胞分类计数的方法。将400例随机选择的医院病例的扫描结果与100个细胞计数的在线结果进行比较。此外，选取50例异常结果百分比高的病例，由四名不同的阅片者分别进行扫描和手工计数。结果表明，在检测未成熟粒细胞和原始细胞等异常细胞类型的存在方面，扫描分类计数与100个细胞的手工计数相当。然而，其正确估计正常细胞，尤其是淋巴细胞相对比例的能力似乎不如手工计数可靠。最重要的是，分析表明，扫描分类计数具有一系列优点和缺点，与新一代自动血液分析仪提供的“三分群分类”技术互补。因此，作者建议将这两种方法结合使用，为手工100个细胞分类计数提供合适的替代方法。
