Islam Mohammad Nazrul, Sultana Shirin, Alam Md Jobaidul
Department of Biotechnology, Sher-e-Bangla Agricultural University, Dhaka 1207, Bangladesh.
Fisheries Biotechnology Division, National Institute of Biotechnology, Dhaka 1349, Bangladesh.
Genomics Inform. 2020 Sep;18(3):e32. doi: 10.5808/GI.2020.18.3.e32. Epub 2020 Sep 10.
The mitochondrial genome of a species is an essential resource for its effective conservation and phylogenetic studies. In this article, we present sequencing and characterization of the complete mitochondrial genome of a threatened labeonine fish, Cirrhinus reba collected from Khulna region of Bangladesh. The complete mitochondrial genome was 16,597 bp in size, which formed a circular double-stranded DNA molecule containing a total of 37 mitochondrial genes (13 protein-coding genes, 2 ribosomal RNA genes, and 22 transfer RNA genes) with two non-coding regions, an origin of light strand replication (OL) and a displacement loop (D-loop), similar structure with other fishes of Teleostei. The phylogenetic tree demonstrated its close relationship with labeonine fishes. The complete mitogenome of Cirrhinus reba (GenBank no. MN862482) showed 99.96% identity to another haplotype of Cirrhinus reba (AP013325), followed by 90.18% identity with Labeo bata (AP011198).
一个物种的线粒体基因组是其有效保护和系统发育研究的重要资源。在本文中，我们展示了从孟加拉国库尔纳地区采集的一种濒危野鲮属鱼类——雷氏野鲮完整线粒体基因组的测序和特征分析。完整的线粒体基因组大小为16,597 bp，形成一个环状双链DNA分子，总共包含37个线粒体基因（13个蛋白质编码基因、2个核糖体RNA基因和22个转移RNA基因），有两个非编码区，一个轻链复制起点（OL）和一个位移环（D-loop），与硬骨鱼纲的其他鱼类结构相似。系统发育树表明它与野鲮属鱼类关系密切。雷氏野鲮的完整线粒体基因组（GenBank编号：MN862482）与雷氏野鲮的另一个单倍型（AP013325）的同一性为99.96%，其次与野鲮（AP011198）的同一性为90.18%。
