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濒危野鲮亚科鱼类瑞氏卷口鱼线粒体全基因组的测序与注释

Sequencing and annotation of the complete mitochondrial genome of a threatened labeonine fish, Cirrhinus reba.

作者信息

Islam Mohammad Nazrul, Sultana Shirin, Alam Md Jobaidul

机构信息

Department of Biotechnology, Sher-e-Bangla Agricultural University, Dhaka 1207, Bangladesh.

Fisheries Biotechnology Division, National Institute of Biotechnology, Dhaka 1349, Bangladesh.

出版信息

Genomics Inform. 2020 Sep;18(3):e32. doi: 10.5808/GI.2020.18.3.e32. Epub 2020 Sep 10.

Abstract

The mitochondrial genome of a species is an essential resource for its effective conservation and phylogenetic studies. In this article, we present sequencing and characterization of the complete mitochondrial genome of a threatened labeonine fish, Cirrhinus reba collected from Khulna region of Bangladesh. The complete mitochondrial genome was 16,597 bp in size, which formed a circular double-stranded DNA molecule containing a total of 37 mitochondrial genes (13 protein-coding genes, 2 ribosomal RNA genes, and 22 transfer RNA genes) with two non-coding regions, an origin of light strand replication (OL) and a displacement loop (D-loop), similar structure with other fishes of Teleostei. The phylogenetic tree demonstrated its close relationship with labeonine fishes. The complete mitogenome of Cirrhinus reba (GenBank no. MN862482) showed 99.96% identity to another haplotype of Cirrhinus reba (AP013325), followed by 90.18% identity with Labeo bata (AP011198).

摘要

一个物种的线粒体基因组是其有效保护和系统发育研究的重要资源。在本文中,我们展示了从孟加拉国库尔纳地区采集的一种濒危野鲮属鱼类——雷氏野鲮完整线粒体基因组的测序和特征分析。完整的线粒体基因组大小为16,597 bp,形成一个环状双链DNA分子,总共包含37个线粒体基因(13个蛋白质编码基因、2个核糖体RNA基因和22个转移RNA基因),有两个非编码区,一个轻链复制起点(OL)和一个位移环(D-loop),与硬骨鱼纲的其他鱼类结构相似。系统发育树表明它与野鲮属鱼类关系密切。雷氏野鲮的完整线粒体基因组(GenBank编号:MN862482)与雷氏野鲮的另一个单倍型(AP013325)的同一性为99.96%,其次与野鲮(AP011198)的同一性为90.18%。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0cb/7560443/2f724453f499/gi-2020-18-3-e32f1.jpg

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