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用于快速检测A群链球菌的新型颜色测试。

Novel color test for rapid detection of group A streptococci.

作者信息

Hadfield S G, Petts D N, Kennedy P, Lane A, McIllmurray M B

出版信息

J Clin Microbiol. 1987 Jul;25(7):1151-4. doi: 10.1128/jcm.25.7.1151-1154.1987.

DOI:10.1128/jcm.25.7.1151-1154.1987
PMID:3301887
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC269165/
Abstract

A novel color test for the rapid detection of group A streptococci has been developed. The test, designed to be suitable for use in clinical laboratories as well as by less experienced personnel, incorporates the simplicity of latex tests with a color change to indicate the presence of group A streptococcal antigen. The test, which takes 5 min, was evaluated with 646 throat swabs, with a 15.6% incidence of group A streptococci; for swabs which yielded 10 or more group A streptococcal colonies in cultures, the sensitivity was 96.8%, and the specificity was 99.1%. In addition, the color test was 100% sensitive and specific when used to detect group A streptococcal antigen in beta-hemolytic colonies from culture plates.

摘要

已开发出一种用于快速检测A群链球菌的新型颜色试验。该试验设计得既适合临床实验室使用,也适合经验较少的人员使用,它结合了乳胶试验的简便性,并通过颜色变化来指示A群链球菌抗原的存在。该试验耗时5分钟,用646份咽拭子进行了评估,A群链球菌的发生率为15.6%;对于培养物中产生10个或更多A群链球菌菌落的咽拭子,敏感性为96.8%,特异性为99.1%。此外,当用于检测培养平板上β溶血菌落中的A群链球菌抗原时,颜色试验的敏感性和特异性均为100%。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f83/269165/fe3a666dcca3/jcm00091-0030-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f83/269165/fe3a666dcca3/jcm00091-0030-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f83/269165/fe3a666dcca3/jcm00091-0030-a.jpg

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Cochrane Database Syst Rev. 2016 Jul 4;7(7):CD010502. doi: 10.1002/14651858.CD010502.pub2.
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Direct detection of groups A, C and G streptococci in clinical specimens by a trivalent colour test.通过三价颜色试验直接检测临床标本中的A、C和G组链球菌。
Eur J Clin Microbiol Infect Dis. 1988 Feb;7(1):34-9. doi: 10.1007/BF01962168.

本文引用的文献

1
Fluorescent-Antibody Identification of Group A Streptococci from Throat Swabs.咽喉拭子中A群链球菌的荧光抗体鉴定
Am J Public Health Nations Health. 1963 Jul;53(7):1083-92. doi: 10.2105/ajph.53.7.1083.
2
Perplexity and precision in the diagnosis of streptococcal pharyngitis.链球菌性咽炎诊断中的困惑与精准度
Am J Dis Child. 1972 Sep;124(3):352-8. doi: 10.1001/archpedi.1972.02110150050009.
3
Beta-hemolytic streptococcal infection. The clinical and epidemiologic importance of the number of organisms found in cultures.
β-溶血性链球菌感染。培养物中发现的微生物数量的临床和流行病学意义。
Am J Dis Child. 1970 Jan;119(1):18-26.
4
Simplified extraction procedure for serological grouping of beta-hemolytic streptococci.β-溶血性链球菌血清学分型的简化提取程序
Appl Microbiol. 1974 Nov;28(5):836-9. doi: 10.1128/am.28.5.836-839.1974.
5
Effect of antibiotic therapy on the clinical course of streptococcal pharyngitis.抗生素治疗对链球菌性咽炎临床病程的影响。
J Pediatr. 1985 Jun;106(6):870-5. doi: 10.1016/s0022-3476(85)80228-6.
6
Evaluation of Detect-A-Strep and the Culturette Ten-Minute Strep ID kits for detection of group A streptococcal antigen in oropharyngeal swabs from children.评估Detect-A-Strep和Culturette十分钟链球菌鉴定试剂盒用于检测儿童咽拭子中A组链球菌抗原的效果。
J Clin Microbiol. 1985 Aug;22(2):145-8. doi: 10.1128/jcm.22.2.145-148.1985.
7
Effect of delay in processing on the performance of Directigen for the detection of group A streptococci in throat swabs.处理延迟对Directigen检测咽拭子中A组链球菌性能的影响。
J Clin Microbiol. 1985 Jun;21(6):993-4. doi: 10.1128/jcm.21.6.993-994.1985.
8
Streptococcal pharyngitis: optimal site for throat culture.
J Pediatr. 1985 May;106(5):781-3. doi: 10.1016/s0022-3476(85)80354-1.
9
Comparison of a latex agglutination test and four culture methods for identification of group A streptococci in a pediatric office laboratory.儿科门诊实验室中乳胶凝集试验与四种培养方法用于鉴定A群链球菌的比较。
J Pediatr. 1986 Mar;108(3):347-51. doi: 10.1016/s0022-3476(86)80871-x.