Petts D N, Lane A, Kennedy P, Hadfield S G, McIllmurray M B
Wellcome Research Laboratory, Beckenham, Kent, UK.
Eur J Clin Microbiol Infect Dis. 1988 Feb;7(1):34-9. doi: 10.1007/BF01962168.
A simple trivalent colour test, developed for the rapid detection and identification of streptococci belonging to Lancefield groups A, C and G, was evaluated for sensitivity and specificity with cultures and when directly used with wound and throat swabs. In tests performed on cultures, all of 94 group A, 78 group C and 94 group G cultures were correctly identified. In direct tests on wound swabs, 49 of 52 group A, 17 of 19 group C and 48 of 51 group G streptococci were detected and correctly identified; no false positives were observed. With throat swabs from pharyngitis patients 34 of 36 group A, 3 of 6 group C and 5 of 8 group G streptococci gave positive results. Almost 10% of these swabs gave false positive reactions with the group C component of the test system. Samples taken from uninfected individuals indicated that the false positives were probably associated with blood group A. The test system gives rapid and reliable results with streptococcal cultures, but when directly applied to clinical samples the results must be interpreted with caution, particularly if the patient's blood group is not known.
一种用于快速检测和鉴定A、C和G群兰氏链球菌的简单三价颜色试验，针对培养物以及直接用于伤口和咽喉拭子时的敏感性和特异性进行了评估。在对培养物进行的测试中，94株A群、78株C群和94株G群培养物全部被正确鉴定。在对伤口拭子进行的直接测试中，52株A群链球菌中的49株、19株C群链球菌中的17株和51株G群链球菌中的48株被检测到并正确鉴定；未观察到假阳性。对于咽炎患者的咽喉拭子，36株A群链球菌中的34株、6株C群链球菌中的3株和8株G群链球菌中的5株呈阳性结果。这些拭子中近10%与测试系统的C群成分出现假阳性反应。从未感染个体采集的样本表明，假阳性可能与A型血有关。该测试系统对链球菌培养物能给出快速可靠的结果，但直接应用于临床样本时，结果必须谨慎解读，尤其是在患者血型未知的情况下。
