Department Institute of Marine Research, NO-5817 Bergen, Norway.
Department of Adipose Tissue Biology, Institute of Physiology Academy of Sciences of the Czech Republic, 14220 Prague, Czech Republic.
Nutrients. 2020 Oct 3;12(10):3038. doi: 10.3390/nu12103038.
Fillets from marine fish species contain n-3 polyunsaturated fatty acids (PUFAs) in the form of phospholipids (PLs). To investigate the importance of PL-bound n-3 PUFAs in mediating the anti-obesogenic effect of lean seafood, we compared the anti-obesogenic properties of fillets from cod with fillets from pangasius, a fresh water fish with a very low content of PL-bound n-3 PUFAs. We prepared high-fat/high-protein diets using chicken, cod and pangasius as the protein sources, and fed male C57BL/6J mice these diets for 12 weeks. Mice fed the diet containing cod gained less adipose tissue mass and had smaller white adipocytes than mice fed the chicken-containing diet, whereas mice fed the pangasius-containing diet were in between mice fed the chicken-containing diet and mice fed the cod-containing diet. Of note, mice fed the pangasius-containing diet exhibited reduced glucose tolerance compared to mice fed the cod-containing diet. Although the sum of marine n-3 PUFAs comprised less than 2% of the total fatty acids in the cod-containing diet, this was sufficient to significantly increase the levels of eicosapentaenoic acid (EPA) and docosahexaenoic acids (DHA) in mouse tissues and enhance production of n-3 PUFA-derived lipid mediators as compared with mice fed pangasius or chicken.
来自海洋鱼类的鱼片以磷脂（PL）的形式含有 n-3 多不饱和脂肪酸（PUFA）。为了研究 PL 结合的 n-3 PUFAs 在介导瘦海鲜的抗肥胖作用中的重要性，我们比较了鳕鱼和 Pangasius（一种 PL 结合的 n-3 PUFAs 含量非常低的淡水鱼）鱼片的抗肥胖特性。我们使用鸡肉、鳕鱼和 Pangasius 作为蛋白质来源，用这些蛋白质来源制备高脂肪/高蛋白饮食，并将这些饮食喂养雄性 C57BL/6J 小鼠 12 周。与喂食含鸡肉饮食的小鼠相比，喂食含鳕鱼饮食的小鼠获得的脂肪组织质量较少，白色脂肪细胞较小，而喂食含 Pangasius 饮食的小鼠则介于喂食含鸡肉饮食的小鼠和喂食含鳕鱼饮食的小鼠之间。值得注意的是，与喂食含鳕鱼饮食的小鼠相比，喂食含 Pangasius 饮食的小鼠的葡萄糖耐量降低。尽管鳕鱼饮食中海洋 n-3 PUFAs 的总和不到总脂肪酸的 2%，但这足以显著增加 EPA 和 DHA 在小鼠组织中的水平，并增强 n-3 PUFAs 衍生的脂质介质的产生，与喂食 Pangasius 或鸡肉的小鼠相比。
