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用于口蹄疫病毒非结构蛋白血清学的 2 种 ELISA 试剂盒的实验室间比较。

Inter-laboratory comparison of 2 ELISA kits used for foot-and-mouth disease virus nonstructural protein serology.

机构信息

The Pirbright Institute, Pirbright, Surrey, United Kingdom.

Animal and Plant Health Agency, New Haw, United Kingdom.

出版信息

J Vet Diagn Invest. 2020 Nov;32(6):933-937. doi: 10.1177/1040638720962070. Epub 2020 Oct 7.

DOI:10.1177/1040638720962070
PMID:33025858
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7645616/
Abstract

Serologic assays used to detect antibodies to nonstructural proteins (NSPs) of foot-and-mouth disease virus (FMDV) are used for disease surveillance in endemic countries, and are essential to providing evidence for freedom of the disease with or without vaccination and to recover the free status of a country after an outbreak. In a 5-site inter-laboratory study, we compared the performance of 2 commercial NSP ELISA kits (ID Screen FMD NSP ELISA single day [short] and overnight protocols, ID.Vet; PrioCHECK FMDV NS antibody ELISA, Thermo Fisher Scientific). The overall concordance between the PrioCHECK and ID Screen test was 93.8% (95% CI: 92.0-95.2%) and 94.8% (95% CI: 93.1-96.1%) for the overnight and short ID Screen incubation protocols, respectively. Our results indicate that the assays (including the 2 different formats of the ID Screen test) can be used interchangeably in post-outbreak serosurveillance.

摘要

用于检测口蹄疫病毒（FMDV）非结构蛋白（NSPs）抗体的血清学检测方法被用于流行国家的疾病监测，对于提供有无疫苗接种的疾病自由证据以及在疫情爆发后恢复国家的自由状态至关重要。在一个 5 个地点的实验室间研究中，我们比较了 2 种商业 NSP ELISA 试剂盒（ID Screen FMD NSP ELISA 单日[短]和隔夜方案，ID.Vet；PrioCHECK FMDV NS 抗体 ELISA，赛默飞世尔科技）的性能。PrioCHECK 和 ID Screen 检测之间的总体一致性分别为 93.8%（95%CI：92.0-95.2%）和 94.8%（95%CI：93.1-96.1%），用于隔夜和 ID Screen 短孵育方案。我们的结果表明，这些检测方法（包括 ID Screen 检测的 2 种不同格式）可在疫情后血清学监测中互换使用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/83ce/7649531/81f570e7ff8e/10.1177_1040638720962070-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/83ce/7649531/a325c7455480/10.1177_1040638720962070-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/83ce/7649531/0a9360cd7dee/10.1177_1040638720962070-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/83ce/7649531/81f570e7ff8e/10.1177_1040638720962070-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/83ce/7649531/a325c7455480/10.1177_1040638720962070-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/83ce/7649531/0a9360cd7dee/10.1177_1040638720962070-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/83ce/7649531/81f570e7ff8e/10.1177_1040638720962070-fig3.jpg

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本文引用的文献

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Comparative evaluation of non-structural protein-antibody detecting ELISAs for foot-and-mouth disease sero-surveillance under intensive vaccination.在强化疫苗接种情况下用于口蹄疫血清学监测的非结构蛋白抗体检测酶联免疫吸附测定法的比较评估
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德国分散型口蹄疫病毒诊断技术的效力证明。
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Comparison of sensitivity and specificity in three commercial foot-and-mouth disease virus non-structural protein ELISA kits with swine sera in Taiwan.台湾地区三种商用口蹄疫病毒非结构蛋白酶联免疫吸附测定试剂盒与猪血清的敏感性和特异性比较
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