Max Planck Tandem Group in Nanobioengineering, University of Antioquia, Complejo Ruta N, Calle 67 N° 52-20, Medellín, 050010, Colombia.
Mikrochim Acta. 2020 Oct 7;187(11):594. doi: 10.1007/s00604-020-04568-1.
The development of a stable nanobioconjugate based on gold nanoparticles (AuNPs) linked to single-strand DNA (ssDNA) is reported for amplification of the electrochemical signal of a Zika virus (ZIKV) genetic material-based bioassay, with high sensitivity. The genosensor was assembled either at a screen-printed gold electrode (SPAuE) or a screen-printed carbon electrode decorated with hierarchical gold nanostructures (SPCE/Au), with Ru as an electrochemical reporter. The genosensor response, interrogated by differential pulse voltammetry (DPV) at the transient current density, was linear from 10 to 600 fM and from 500 fM to 10 pM of the target, with a sensitivity of 2.7 and 2.9 μA cm M and a limit of detection of 0.2 and 33 fM at the SPAuE and SPCE/Au, respectively. The resultant genosensor detected ZIKV genetic material in raw serum samples from infected patients, with no sample pretreatment in a polymerase chain reaction amplification-free assay. The proposed ultrasensitive nanobioconjugate-based system offers a step forward to the diagnosis of the ZIKV, closer to the patient, and holds the potential for signal amplification in biosensing of a myriad of applications.Graphical abstract.
据报道，一种基于金纳米粒子（AuNPs）与单链 DNA（ssDNA）连接的稳定纳米生物缀合物的发展，可用于放大寨卡病毒（ZIKV）基于遗传物质的生物测定的电化学信号，具有高灵敏度。该基因传感器可以在丝网印刷金电极（SPAuE）或用分层金纳米结构修饰的丝网印刷碳电极（SPCE/Au）上组装，其中 Ru 用作电化学报告器。通过差分脉冲伏安法（DPV）在瞬态电流密度下检测基因传感器的响应，在目标物为 10 至 600 fM 和 500 fM 至 10 pM 的范围内呈线性，在 SPAuE 和 SPCE/Au 上的灵敏度分别为 2.7 和 2.9 μA cm M 和检测限分别为 0.2 和 33 fM。该基因传感器可在未经聚合酶链反应扩增的无样品预处理的情况下，从感染患者的原始血清样本中检测到 ZIKV 遗传物质。所提出的基于超灵敏纳米生物缀合物的系统为寨卡病毒的诊断提供了一个新的进展，更接近患者，并且在生物传感的众多应用中具有信号放大的潜力。
