Longhua Hospital Shanghai University of Traditional Chinese Medicine, Shanghai, 200032, China.
Shanghai Pu Dong Hospital, Shanghai, China.
J Ethnopharmacol. 2021 Feb 10;266:113442. doi: 10.1016/j.jep.2020.113442. Epub 2020 Oct 4.
Qingchang Suppository (QCS) is a Traditional Chinese Medicine formula (TCM) for Ulcerative Colitis (UC), which has been used for the treatment of UC for more than 30 years with therapeutic effect. This formula is optimized from a classic formula called "Qingdai San". Although some experiments have shown QCS effective for UC, its mechanism on UC is still unclear and needs to be clarified.
To investigate the usage of QCS in our hospital, clarify the main compounds in QCS and their anti-inflammation effect both in vivo and in vitro.
Prescription analysis was performed in the clinical department and pharmacology network prediction was predicted for relative signal pathways. 2,4,6-Trinitrobenzenesulfonic acid (TNBS)-induced colitis rats and Lipopolysaccharide (LPS)-induced Caco-2 cell as an inflammatory model were used to evaluate the effect of QCS.
QCS and its herbs were associated with inflammatory and immunological diseases. QCS and its ingredients showed little toxicity on Caco-2 cell and could down-regulate the level of Interleukin-6 (IL-6) and expression of signal transducer and activator of transcription 3 (P-STAT3 Tyr705) in LPS-induced Caco-2 cell. In an animal experiment, QCS and its ingredients (indigo and gallic acid) could alleviate the symptoms of TNBS-induced colitis of rats, significantly decrease pro-inflammatory factors and anti-inflammatory factors as well as inhibit the expressions of P-STAT3 and Tyr705.
QCS and its components could improve UC by anti-inflammation. JAK2/STAT3 pathway might be the possible signaling pathway.
清肠栓（QCS）是一种用于溃疡性结肠炎（UC）的中药配方（TCM），已用于治疗 UC 超过 30 年，具有治疗效果。该配方是从一种名为“清黛散”的经典配方优化而来。虽然一些实验表明 QCS 对 UC 有效，但它对 UC 的作用机制尚不清楚，需要进一步阐明。
调查我院 QCS 的使用情况，阐明 QCS 的主要化合物及其在体内和体外的抗炎作用。
在临床科室进行处方分析，并通过药理学网络预测相对信号通路。采用 2,4,6-三硝基苯磺酸（TNBS）诱导的结肠炎大鼠和脂多糖（LPS）诱导的 Caco-2 细胞作为炎症模型，评价 QCS 的作用。
QCS 及其草药与炎症性和免疫性疾病有关。QCS 及其成分对 Caco-2 细胞几乎没有毒性，可下调 LPS 诱导的 Caco-2 细胞中白细胞介素 6（IL-6）的水平和信号转导和转录激活因子 3（P-STAT3 Tyr705）的表达。在动物实验中，QCS 及其成分（靛蓝和没食子酸）可缓解 TNBS 诱导的结肠炎大鼠的症状，显著降低促炎因子和抗炎因子，并抑制 P-STAT3 和 Tyr705 的表达。
QCS 及其成分可通过抗炎作用改善 UC。JAK2/STAT3 通路可能是其潜在的信号通路。
