Department of Analytical Biochemistry, Institute of Food Technology and Nutrition, College of Natural Sciences, Rzeszow University, Zelwerowicza Street 4, 35-601 Rzeszow, Poland.
Department of Bioenergetics, Food Analysis and Microbiology, Institute of Food Technology and Nutrition, College of Natural Sciences, Rzeszow University, Zelwerowicza Street 4, 35-601 Rzeszow, Poland.
Oxid Med Cell Longev. 2020 Sep 23;2020:6423783. doi: 10.1155/2020/6423783. eCollection 2020.
Replicative senescence is an unalterable growth arrest of primary cells in the culture system. It has been reported that aging is related to the limited replicative capacity that normal somatic cells show . If oxidative damage contributes to the lifespan limitation, antioxidants are expected to extend the replicative lifespan of fibroblasts. This article critically reviews the results of experiments devoted to this problem performed within the last decades under conditions of culture. The results of studied are heterogeneous, some papers showing no effects of antioxidants; most finding limited enhancement of reproductive capacity of fibroblasts, some reporting a significant extension of replicative lifespan (RLS). Both natural and synthetic antioxidants were found to extend the RLS of fibroblasts, either by a direct antioxidant effect or, indirectly, by activation of signaling pathways and activation of proteasomes or hormetic effects. Most significant prolongation of RLS was reported so far for nicotinamide, -hydroxylamines, carnosine and Methylene Blue. These results may be of importance for the design of skin-protecting cosmetics.
复制性衰老（Replicative senescence）是原代细胞在培养体系中不可改变的生长停滞。据报道，衰老与正常体细胞表现出的有限复制能力有关。如果氧化损伤导致寿命限制，那么抗氧化剂有望延长成纤维细胞的复制寿命。本文批判性地回顾了过去几十年在培养条件下专门针对该问题进行的实验结果。研究结果存在异质性，一些论文显示抗氧化剂没有效果；大多数发现成纤维细胞的生殖能力有限提高，一些报告说复制寿命（RLS）显著延长。天然和合成抗氧化剂都被发现可以延长成纤维细胞的 RLS，或者通过直接的抗氧化作用，或者通过激活信号通路和激活蛋白酶体或应激效应来间接延长。迄今为止，烟酰胺、-羟基胺、肌肽和亚甲蓝报告的 RLS 延长最显著。这些结果对于设计皮肤保护化妆品可能很重要。
