Ye Zhou, Kobe Alexandra C, Sang Ting, Aparicio Conrado
MDRCBB, Minnesota Dental Research Center for Biomaterials and Biomechanics, University of Minnesota, Minneapolis, Minnesota 55455, USA.
Nanoscale. 2020 Oct 22;12(40):20767-20775. doi: 10.1039/d0nr04526h.
With the increasing threat from antibiotic-resistant bacteria, surface modification with antimicrobial peptides (AMP) has been promisingly explored for preventing bacterial infections. Little is known about the critical factors that govern AMP-surface interactions to obtain stable and active coatings. Here, we systematically monitored the adsorption of a designer amphipathic AMP, GL13K, on model surfaces. Self-assembly of the GL13K peptides formed supramolecular amphiphiles that highly adsorbed on negatively charged, polar hydroxyapatite-coated sensors. We further tuned surface charge and/or surface polarity with self-assembled monolayers (SAMs) on Au sensors and studied their interactions with adsorbed GL13K. We determined that the surface polarity of the SAM-coated sensors instead of their surface charge was the dominant factor governing AMP/substrate interactions via hydrogen bonding. Our findings will instruct the universal design of efficient self-assembled AMP coatings on biomaterials, biomedical devices and/or natural tissues.
随着抗生素耐药菌带来的威胁日益增加，利用抗菌肽（AMP）进行表面改性已成为预防细菌感染的一种很有前景的探索方向。对于决定AMP与表面相互作用以获得稳定且具活性涂层的关键因素，我们了解甚少。在此，我们系统地监测了一种设计合成的两亲性AMP（GL13K）在模型表面的吸附情况。GL13K肽的自组装形成了超分子两亲物，其高度吸附在带负电荷的极性羟基磷灰石涂层传感器上。我们进一步通过在金传感器上的自组装单分子层（SAMs）来调节表面电荷和/或表面极性，并研究它们与吸附的GL13K之间的相互作用。我们确定，SAM涂层传感器的表面极性而非其表面电荷是通过氢键决定AMP/底物相互作用的主导因素。我们的研究结果将指导在生物材料、生物医学设备和/或天然组织上高效自组装AMP涂层的通用设计。
