Ankara University Faculty of Science Department of Biology, 06100, Tandogan, Ankara, Turkey.
Evolutionary Genetics Laboratory (eGL), Ankara University Faculty of Agriculture Department of Fisheries and Aquaculture, 06110, Dışkapı, Ankara, Turkey.
Virology. 2021 Jan 2;552:10-19. doi: 10.1016/j.virol.2020.09.009. Epub 2020 Oct 3.
Viruses hosted by ectomycorrhizal fungi remain poorly studied. In this study, we detected eight new fungal viruses co-infecting a single isolate of the hypogeous ectomycorrhizal fungus Picoa juniperi using high-throughput sequencing. Phylogenetic analysis of one identified virus abbreviated as PjMTV1 revealed its closest relatives as members of the newly proposed family "Megatotiviridae". Phylogenetic analyses of two identified viruses abbreviated as PjV1 and PjV2 showed that these viruses are associated with members of the proposed family "Fusagraviridae". Phylogenetic analysis of the identified one another virus abbreviated as PjYV1 demonstrated that this virus is related to the members of the proposed family Yadokariviridae. The remaining four identified virus-like contigs were determined as segments of the bipartite dsRNA mycoviruses from the family Partitiviridae. The mycoviruses reported in this study are the first viruses described in Picoa juniperi, and PjMTV1 characterized herein is the secondly reported member of the newly proposed family "Megatotiviridae".
外生菌根真菌携带的病毒研究甚少。本研究采用高通量测序技术，检测到一株地下外生菌根真菌 Juniperomyces picoe 单一分离物中同时感染的 8 种新真菌病毒。对鉴定出的一种病毒缩写为 PjMTV1 的系统发育分析表明，其最接近的亲缘病毒是新提出的“Megatotiviridae”家族的成员。鉴定出的两种病毒缩写为 PjV1 和 PjV2 的系统发育分析表明，这些病毒与提出的“Fusagraviridae”家族的成员有关。鉴定出的另一种病毒缩写为 PjYV1 的系统发育分析表明，该病毒与提出的 Yadokariviridae 家族的成员有关。其余鉴定出的四个类似病毒的连续序列被确定为二分体 dsRNA 真菌病毒家族 Partitiviridae 的片段。本研究报道的真菌病毒是在 Juniperomyces picoe 中首次描述的病毒，本文中描述的 PjMTV1 是新提出的“Megatotiviridae”家族的第二个成员。
