Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, China.
J Food Biochem. 2019 May;43(5):e12827. doi: 10.1111/jfbc.12827. Epub 2019 Mar 21.
In this work, alcalase and flavorzyme were chosen as the hydrolase for preparing high Fischer ratio oligopeptides from Antarctic krill (Euphausia superba) (HFP) using sequential enzyme hydrolysis process, and their hydrolysis conditions were optimized using single factor experiment. According to the Fischer ratio, granular activated carbon of XHJ-200 (200 mesh) showed the best way to remove aromatic amino acids and its optimal parameters were pH value of 6.0, adsorption time of 2.5 hr, temperature of 25°C, and solid-liquid ratio of 1:20. The Fischer ratio and average molecular weight of HFP were 21.12 (>20) and 779.9 Da, respectively. In addition, the peptide profile of HFP was established using RP-HPLC and 23 oligopeptides isolated from HFP including 6 dipeptides, 9 tripeptides, 3 tetrapeptides, and 5 pentapeptides were identified using protein amino acid sequence analyzer and mass spectrum. Furthermore, HFP exhibited high radical scavenging activity, reducing power, and lipid peroxidation inhibition capability. PRACTICAL APPLICATIONS: High Fischer ratio oligopeptides (HFO) is a kind of small peptide mixture with the mole ratio of branched-chain amino acids (BCAA) to aromatic amino acids (AAA) higher than 20, which has drawn a great attention due to its great potential in clinical nutrition approaches for the treatment of liver disease when amino acid composition is out of proportion characterized by low levels of BCAA and high levels of AAA in the systemic blood. Antarctic krill is regarded as the largest animal protein resource for various food and pharmaceutical products. However, there is no report on the preparation of HFO of Antarctic krill (HFP). Therefore, the aim of the present study was to investigate the preparation process, peptide profiles and in vitro antioxidant activity of HFP. This study will potentially enhance the value-added utilization of Antarctic krill by making it an important raw material in the health-promoting functional products.
在这项工作中，选用了碱性蛋白酶和风味蛋白酶作为水解酶，通过顺序酶解的方法从南极磷虾（Euphausia superba）中制备高 Fischer 比寡肽（HFP），并通过单因素实验对其水解条件进行了优化。根据 Fischer 比，XHJ-200（200 目）颗粒活性炭去除芳香族氨基酸的效果最好，其最佳参数为 pH 值 6.0、吸附时间 2.5 小时、温度 25°C、固液比 1:20。HFP 的 Fischer 比和平均分子量分别为 21.12（>20）和 779.9 Da。此外，还采用 RP-HPLC 建立了 HFP 的肽谱，从 HFP 中分离出 23 种寡肽，其中 6 种二肽、9 种三肽、3 种四肽和 5 种五肽通过蛋白质氨基酸序列分析仪和质谱进行了鉴定。此外，HFP 还表现出较高的自由基清除活性、还原能力和脂质过氧化抑制能力。
高 Fischer 比寡肽（HFO）是一种由支链氨基酸（BCAA）与芳香族氨基酸（AAA）的摩尔比大于 20 的小肽混合物，由于其在临床营养治疗肝病方面的巨大潜力而备受关注，当氨基酸组成比例失调时，血液中 BCAA 水平低而 AAA 水平高。南极磷虾被认为是各种食品和药品的最大动物蛋白资源。然而，目前尚无关于南极磷虾（HFP）高 Fischer 比寡肽制备的报道。因此，本研究旨在探讨 HFP 的制备工艺、肽谱及体外抗氧化活性。本研究将有可能通过将南极磷虾作为重要的原料，提高其在促进健康的功能性产品中的附加值利用。
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