Key Laboratory for Biorheological Science and Technology (Chongqing University), Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, 400044, People's Republic of China.
Liquor Making Biology Technology and Application of Key Laboratory of Sichuan Province, College of Bioengineering, Sichuan University of Science and Engineering, Zigong, 643000, People's Republic of China.
Mikrochim Acta. 2020 Oct 9;187(11):601. doi: 10.1007/s00604-020-04565-4.
Hemin as the organic linker ligand and Cu (II) as the metal center were applied to prepare a copper-metal-organic framework (Cu-hemin-MOF) via one-step hydrothermal method. Characterization using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and X-ray powder diffraction (XRD) demonstrate that the acquired Cu-hemin-MOF possesses the appearance of 3D ball-flower shape with the existence of C, N, O, Fe, and Cu on the surface. Further study found that this 3D ball-flower shaped Cu-hemin-MOF exhibits peroxidase-like activity, which can catalyze the peroxidase substrate of o-phenylenediamine (OPD) to generate 2,3-diaminophenazine (DAP) in the presence of HO. DAP has a yellow color and also emits a strong fluorescence when excited by ultraviolet light. Interestingly, Cu-hemin-MOF's peroxidase-like activity can be strongly inhibited by glutathione (GSH). In view of this, a dual readout (fluorescence and colorimetry) was proposed to detect GSH for the first time. Under optimal conditions, the proposed method exhibits good linear relationship between the signal response (fluorescence and colorimetry) and the concentration of GSH, and low limits of detection (LOD) of 2.3 and 26.6 nM, respectively.Graphical abstract.
血红素作为有机连接配体，Cu(II)作为金属中心，通过一步水热法制备了铜金属有机骨架（Cu-血红素-MOF）。采用扫描电子显微镜（SEM）、傅里叶变换红外光谱（FT-IR）、X 射线光电子能谱（XPS）和 X 射线粉末衍射（XRD）对其进行了表征，结果表明所获得的 Cu-血红素-MOF 具有 3D 球花状外观，表面存在 C、N、O、Fe 和 Cu。进一步研究发现，这种 3D 球花状的 Cu-血红素-MOF 具有过氧化物酶样活性，能够在 HO 的存在下催化邻苯二胺（OPD）的过氧化物酶底物生成 2,3-二氨基吩嗪（DAP）。DAP 呈黄色，在紫外光激发下也会发出强烈的荧光。有趣的是，Cu-血红素-MOF 的过氧化物酶样活性可以被谷胱甘肽（GSH）强烈抑制。鉴于此，首次提出了一种双读取（荧光和比色法）来检测 GSH。在最佳条件下，该方法的信号响应（荧光和比色法）与 GSH 浓度之间呈良好的线性关系，检测限（LOD）分别为 2.3 和 26.6 nM。
