Roman-Reyna Veronica, Pinili Dale, Borja Frances N, Quibod Ian L, Groen Simon C, Alexandrov Nickolai, Mauleon Ramil, Oliva Ricardo
Rice Breeding Platform, International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines.
Present Address: Department of Plant Pathology, The Ohio State University, Columbus, OH, USA.
Rice (N Y). 2020 Oct 9;13(1):72. doi: 10.1186/s12284-020-00432-1.
The crop microbial communities are shaped by interactions between the host, microbes and the environment, however, their relative contribution is beginning to be understood. Here, we explore these interactions in the leaf bacterial community across 3024 rice accessions.
By using unmapped DNA sequencing reads as microbial reads, we characterized the structure of the rice bacterial microbiome. We identified central bacteria taxa that emerge as microbial "hubs" and may have an influence on the network of host-microbe interactions. We found regions in the rice genome that might control the assembly of these microbial hubs. To our knowledge this is one of the first studies that uses raw data from plant genome sequencing projects to characterize the leaf bacterial communities.
We showed, that the structure of the rice leaf microbiome is modulated by multiple interactions among host, microbes, and environment. Our data provide insight into the factors influencing microbial assemblage in the rice leaf and also opens the door for future initiatives to modulate rice consortia for crop improvement efforts.
作物微生物群落是由宿主、微生物和环境之间的相互作用塑造的，然而，它们的相对贡献才刚刚开始被理解。在这里，我们在3024份水稻种质的叶片细菌群落中探索这些相互作用。
通过将未映射的DNA测序读数用作微生物读数，我们对水稻细菌微生物组的结构进行了表征。我们鉴定出了作为微生物“枢纽”出现的核心细菌类群，它们可能对宿主-微生物相互作用网络有影响。我们在水稻基因组中发现了可能控制这些微生物枢纽组装的区域。据我们所知，这是首批利用植物基因组测序项目的原始数据来表征叶片细菌群落的研究之一。
我们表明，水稻叶片微生物组的结构受到宿主、微生物和环境之间多种相互作用的调节。我们的数据为影响水稻叶片中微生物组装的因素提供了见解，也为未来调节水稻共生体以进行作物改良的举措打开了大门。
