Handrick Susann, Bestehorn-Willmann Malena, Eckstein Simone, Walter Mathias C, Antwerpen Markus H, Naija Habiba, Stoecker Kilian, Wölfel Roman, Ben Moussa Mohamed
Bundeswehr Institute of Microbiology, Munich, Germany.
Department of Virology, Military Hospital of Instruction of Tunis, Tunis, Tunisia.
Virus Genes. 2020 Dec;56(6):767-771. doi: 10.1007/s11262-020-01795-9. Epub 2020 Oct 9.
In the present work, two complete genome sequences of SARS-CoV-2 were obtained from nasal swab samples of Tunisian SARS-CoV-2 PCR-positive patients using nanopore sequencing. The virus genomes of two of the patients examined, a Tunisian soldier returning from a mission in Morocco and a member of another Tunisian family, showed significant differences in analyses of the total genome and single nucleotide polymorphisms (SNPs). Phylogenetic relationships with known SARS-CoV-2 genomes in the African region, some European and Middle Eastern countries and initial epidemiological conclusions indicate that the introduction of SARS-CoV-2 into Tunisia from two independent sources was travel-related.
在本研究中，使用纳米孔测序技术从突尼斯SARS-CoV-2 PCR检测呈阳性患者的鼻拭子样本中获得了两个SARS-CoV-2完整基因组序列。在对两名受检患者（一名从摩洛哥执行任务归来的突尼斯士兵和另一个突尼斯家庭的一名成员）的病毒基因组进行全基因组和单核苷酸多态性（SNP）分析时，发现存在显著差异。与非洲地区、一些欧洲和中东国家已知的SARS-CoV-2基因组的系统发育关系以及初步的流行病学结论表明，SARS-CoV-2从两个独立来源传入突尼斯与旅行相关。
