The Photonics Laboratory, State Key Laboratory of Applied Optics, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun, Jilin, 130033, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Department of Biomedical Engineering, Texas A&M University, College Station, Texas 77843, United States.
Acta Biomater. 2020 Dec;118:44-53. doi: 10.1016/j.actbio.2020.09.054. Epub 2020 Oct 6.
Over the past few decades, DNA-based computing technology has become a rapidly developing technology and shown remarkable capabilities in handling complex computational problems. However, most of the logical operations that DNA computer can achieve are still very basic or using large-scale operations to realize complex functions, especially in mathematics. Graphene oxide (GO) is an ideal nanomaterial for biological computing, which has been used in our previous work to perform basic logic operations. Here, we utilize GO to implement far more complex and large-scale logical computing. For the first time, in this work, we utilize the unique interaction between GO and a variety of classified single-stranded DNAs as the reaction platform, by segmenting and encoding the DNA sequences, and programming the interactions between inputs and between the inputs and reaction platform, two relative large-scale logic operations, 6-bit square-root and 9-bit cube-root logical circuits are realized. This study provides a simple but efficient method for advanced and large-scale logical mathematic operations in biotechnology, opening a new horizon for building biocomputer-based innovative functional devices.
在过去的几十年中，基于 DNA 的计算技术已经成为一种快速发展的技术，并在处理复杂计算问题方面显示出了显著的能力。然而，DNA 计算机能够实现的大多数逻辑操作仍然非常基础，或者使用大规模操作来实现复杂功能，特别是在数学方面。氧化石墨烯（GO）是生物计算的理想纳米材料，我们之前的工作已经使用它来执行基本的逻辑操作。在这里，我们利用 GO 来实现更复杂和大规模的逻辑计算。在这项工作中，我们首次利用 GO 与各种分类的单链 DNA 之间的独特相互作用作为反应平台，通过对 DNA 序列进行分割和编码，并对输入之间以及输入与反应平台之间的相互作用进行编程，实现了两个相对大规模的逻辑运算，即 6 位平方根和 9 位立方根逻辑电路。这项研究为生物技术中高级和大规模逻辑数学运算提供了一种简单但有效的方法，为构建基于生物计算机的创新功能设备开辟了新的视野。
