Agricultural Bio-resource Research Institute, Fujian Academy of Agriculture Sciences, Fuzhou 350003, China.
Agricultural Bio-resource Research Institute, Fujian Academy of Agriculture Sciences, Fuzhou 350003, China.
Bioresour Technol. 2021 Jan;319:124155. doi: 10.1016/j.biortech.2020.124155. Epub 2020 Sep 24.
In this work, agricultural wastes were treated by composting in an ectopic fermentation bed system (EFBS) with a continuous nitrogen addition technique. With decreasing of NH-N concentration and increasing of NO-N concentration were observed, and activities of protease, urease and nitrate reductase changed significantly during the fermentation process. To elucidate the key microbes and their function in nitrogen-transforming, microbial diversity and clusters of orthologous groups (COGs) in composting materials were evaluated using metagenomic technology. Comparing with ammonification, the COGs involved in nitrification and denitrification were predominant in the composts. The correlation heatmap revealed that Streptomyces predominant in ammonification was significantly affected by contents of N, NH-N and NO-N. Meanwhile, ammonia-oxidizing archaea (AOA) had a positive relationship with moisture. The most abundant genera in denitrification had positive relationships with N and NO-N. The results indicated that EFBS had functionally diverse microbes and COGs for NH removal.
在这项工作中，农业废弃物通过异位发酵床系统（EFBS）与连续氮添加技术进行堆肥处理。在发酵过程中，观察到 NH-N 浓度降低和 NO-N 浓度增加，蛋白酶、脲酶和硝酸还原酶的活性显著变化。为了阐明氮转化过程中的关键微生物及其功能，采用宏基因组技术评估了堆肥材料中的微生物多样性和直系同源群（COG）。与氨化相比，硝化和反硝化过程中涉及的 COG 在堆肥中占优势。相关热图显示，在氨化中占主导地位的链霉菌受 N、NH-N 和 NO-N 含量的显著影响。同时，氨氧化古菌（AOA）与水分呈正相关。反硝化作用中最丰富的属与 N 和 NO-N 呈正相关。结果表明，EFBS 具有功能多样的微生物和 COG 用于 NH 去除。
