Department of Chemistry, University of Oxford, Chemistry Research Laboratory, Mansfield Road, Oxford, OX1 3TA, UK.
Department of Chemistry, University of Oxford, Inorganic Chemistry Laboratory, South Parks Road, Oxford, OX1 3QR, UK.
Angew Chem Int Ed Engl. 2021 Feb 23;60(9):4434-4447. doi: 10.1002/anie.202011468. Epub 2020 Dec 22.
Kinetic resolution using biocatalysis has proven to be an excellent complementary technique to traditional asymmetric catalysis for the production of enantioenriched compounds. Resolution using oxidative enzymes produces valuable oxygenated structures for use in synthetic route development. This Minireview focuses on enzymes which catalyse the insertion of an oxygen atom into the substrate and, in so doing, can achieve oxidative kinetic resolution. The Baeyer-Villiger rearrangement, epoxidation, and hydroxylation are included, and biological advancements in enzyme development, and applications of these key enantioenriched intermediates in natural product synthesis are discussed.
利用生物催化进行动力学拆分已被证明是一种极好的、与传统不对称催化相辅相成的技术，可用于生产手性富集化合物。利用氧化酶进行拆分可以得到有价值的含氧结构，用于合成路线的开发。本综述重点介绍了能够催化氧原子插入底物的酶，从而实现氧化动力学拆分。其中包括 Baeyer-Villiger 重排、环氧化和羟化反应，并讨论了酶开发方面的生物学进展，以及这些关键的手性富集中间体在天然产物合成中的应用。
