Faculty of Medicine Department of Medical Biochemistry, Eskişehir Osmangazi University, Eskişehir, Türkiye.
Training and Research Center, Kütahya Health Science University, Kütahya, Türkiye.
J Invest Surg. 2022 Aug;35(8):1626-1634. doi: 10.1080/08941939.2022.2084189. Epub 2022 Jun 1.
Oral glucose loading may affect oxidative status during oral glucose tolerance test (OGTT). We aimed to investigate how oxidant and antioxidant markers and thiol/disulfide parameters change during OGTT.
OGTT was performed to 42 volunteers who were considered risk of type 2 diabetes and were divided into three groups (normoglycemic, prediabetes, diabetes) according glucose levels during OGTT. Glucose, insulin, c-peptide, total antioxidant status (TAS), total oxidant status (TOS), total thiol and native thiol were investigated with auto-anaylzer for five-hours period.
Decrease of TAS and increase of TOS levels began with the increase in glucose and insulin levels. The increase of TAS started at third hour and reached the highest levels at fifth hour. OSI levels were higher at fourth hour than fasting and first hours in normoglycemic and diabetes groups. In the prediabetic group, TAS were higher than the other groups, TOS peak was at the second hour Native thiol and total thiol levels showed variable course during OGTT, both parameters increased at the end of the process ). Disulfide levels showed an increase trend but it was not statistically different in normoglycemic and diabetes groups. In prediabetes group, second hour disulfide level was lower than fasting state and disulfide was significantly increased at third, fourth and fifth hours and fifth hour disulfide level was also higher than fasting.
Oxidative stress parameters and thiol/disulfide balance were found to deteriorate within five-hours after glucose loading in all groups. These results indicates that oxidative stress occurs during OGTT.
口服葡萄糖负荷可能会影响口服葡萄糖耐量试验（OGTT）期间的氧化状态。我们旨在研究在 OGTT 期间氧化还原标志物和巯基/二硫键参数如何变化。
对 42 名被认为有 2 型糖尿病风险的志愿者进行 OGTT，并根据 OGTT 期间的血糖水平将其分为三组（正常血糖、糖尿病前期、糖尿病）。在五个小时的时间内，使用自动分析仪检测葡萄糖、胰岛素、C 肽、总抗氧化状态（TAS）、总氧化状态（TOS）、总巯基和天然巯基。
TAS 水平的降低和 TOS 水平的升高始于葡萄糖和胰岛素水平的升高。TAS 的增加始于第三个小时，在第五个小时达到最高水平。在正常血糖和糖尿病组中，OSI 水平在第四个小时高于空腹和第一个小时。在糖尿病前期组中，TAS 高于其他组，TOS 峰值出现在第二个小时。天然巯基和总巯基水平在 OGTT 期间呈变化趋势，两个参数在过程结束时均增加。二硫键水平呈增加趋势，但在正常血糖和糖尿病组中无统计学差异。在糖尿病前期组中，第二个小时的二硫键水平低于空腹状态，第三个、第四个和第五个小时的二硫键显著增加，第五个小时的二硫键水平也高于空腹。
在所有组中，在葡萄糖负荷后五个小时内发现氧化应激参数和巯基/二硫键平衡恶化。这些结果表明，在 OGTT 期间发生氧化应激。
